EMAGE mouse gene expression spatial database

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EMAGE:15099

Zyx zyxin ( MGI:103072)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010280. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010280_01	euxassay_010280_02	euxassay_010280_03	euxassay_010280_04

euxassay_010280_05	euxassay_010280_06	euxassay_010280_07	euxassay_010280_08	euxassay_010280_09

euxassay_010280_10	euxassay_010280_11	euxassay_010280_12	euxassay_010280_13	euxassay_010280_14

euxassay_010280_15	euxassay_010280_16	euxassay_010280_17	euxassay_010280_18	euxassay_010280_19

euxassay_010280_20	euxassay_010280_21	euxassay_010280_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15099_wholemount_strong.wlz

15099_wholemount_moderate.wlz

15099_wholemount_weak.wlz

15099_wholemount_possible.wlz

15099_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15099_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

tongue muscle

weak

regional

weak expression: see section 11 12 13 14 15

liver lobe

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

tail mesenchyme

weak

regional

weak expression: see section 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37230
Entity Detected:	Zyx, zyxin ( MGI:103072)
Sequence:	sense strand is shown >T37230 ACTCCAGCACCTAAGTTTGCTCCAGTGGCTCCTAAATTTACTCCCGTGGTTTCCAAGTTCAGCCCTGGTG CTCCAAGTGGACCTGGGCCACAGCCCAATCAAAAAATGGTGCCTCCGGATGCTCCTTCTTCTGTGAGCAC AGGCTCCCCTCAGCCCCCTAGCTTCACCTATGCTCAGCAGAAGGAGAAGCCCCTAGTTCAAGAGAAGCAG CACCCACAGCCTCCACCAGCTCAAAACCAAAACCAGGTACGCTCTCCTGGAGGCCCAGGCCCCTTGACCC TGAAGGAGGTAGAGGAGTTGGAGCAGCTGACCCAGCAGCTGATGCAGGACATGGAACACCCTCAGAGGCA GAGCGTGGCAGTGAATGAGTCCTGTGGCAAATGCAATCAGCCACTGGCCCGTGCACAGCCTGCGGTTCGT GCACTGGGACAACTGTTCCACATCACCTGCTTCACTTGCCATCAGTGTCAGCAGCAGCTGCAGGGACAGC AGTTCTATAGCCTGGAGGGAGCACCATATTGTGAGGGCTGCTACACCGACACTTTGGAGAAGTGCAACAC CTGTGGGCAGCCCATCACTGACCGCATGCTGAGGGCCACTGGCAAAGCCTACCACCCACAGTGCTTCACC TGTGTGGTCTGCGCCTGTCCCCTGGAGGGCACCTCCTTCATTGTGGACCAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 89311. Forward Primer - name:089311_F_cDNA_Zyx, sequence:ACTCCAGCACCTAAGTTTGCTC; Reverse Primer - name:089311_N_SP6_cDNA_Zyx, sequence:CTGGTCCACAATGAAGGAGGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15103 same embryo
	EMAGE:15098 same embryo
	EMAGE:15101 same embryo
	EMAGE:15102 same embryo
	EMAGE:15100 same embryo
	EurExpress:euxassay_010280 same experiment
	MGI:4829373 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source