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EMAGE:15119

Atp11c ATPase, class VI, type 11C ( MGI:1859661)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15119 EMAGE:15119 EMAGE:15119 EMAGE:15119 EMAGE:15119
"Pseudo-wholemount" of euxassay_010318. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010318_01 euxassay_010318_02 euxassay_010318_03 euxassay_010318_04
EMAGE:15119 EMAGE:15119 EMAGE:15119 EMAGE:15119 EMAGE:15119
euxassay_010318_05 euxassay_010318_06 euxassay_010318_07 euxassay_010318_08 euxassay_010318_09
EMAGE:15119 EMAGE:15119 EMAGE:15119 EMAGE:15119 EMAGE:15119
euxassay_010318_10 euxassay_010318_11 euxassay_010318_12 euxassay_010318_13 euxassay_010318_14
EMAGE:15119 EMAGE:15119 EMAGE:15119 EMAGE:15119 EMAGE:15119
euxassay_010318_15 euxassay_010318_16 euxassay_010318_17 euxassay_010318_18 euxassay_010318_19
EMAGE:15119 EMAGE:15119 EMAGE:15119 EMAGE:15119 EMAGE:15119
euxassay_010318_20 euxassay_010318_21 euxassay_010318_22 euxassay_010318_23 euxassay_010318_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15119Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15119_wholemount_strong.wlz
15119_wholemount_moderate.wlz
15119_wholemount_weak.wlz
15119_wholemount_possible.wlz
15119_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15119_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
diencephalon roof plate
moderate moderate
regionalmoderate expression: see section 14
choroid invagination
moderate moderate
regionalmoderate expression: see section 09 10 11 16 17 18 19
metencephalon part of 4th ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37569
Entity Detected:Atp11c, ATPase, class VI, type 11C ( MGI:1859661)
Sequence:sense strand is shown

>T37569
GGATTATCTCAGACTGATGGGCCCTTAGCCTATTTTGATAAAGCAGATAAGAACCGAGAGGCACTCTTTC
TCCGTGCCCTATGCTTATGTCACACTGTAGAAATGAAAACAAATGATGATGTTGATGGACCTGTAGAAGG
AGCCGGATTCACATATATCTCCTCCTCACCAGATGAAATAGCTTTGGTGAAAGGAGCTAAAAGGTTTGGG
TTCACATTTTTGGGAAATCAGAATGGATATATCAGAGTAGAGAACCAAAGAAAAGAAATAGAAGAGTATG
AACTTCTCCACACCTTAAATTTTGATTCTGTCCGCCGACGTATGAGTGTAATTGTAAGGACCCAAAAAGG
AGATATTCTACTTTTCTGTAAAGGAGCAGATTCATCAATTTTTCCCAGGGTACATAGCCATCAAATTGAG
TTAACCAAAGACCATGTGGAACGTAATGCAATGGATGGGTATCGGACTCTTTGTGTAGCCTTCAAAGAAA
TTCCTCCAGATGATTTTGAAAGAATCAATGCACAACTAGTAGAGGCAAAAATGGCCCTACAAGATAGAGA
AGAAAAACTGGAAAAGGTTTTTGATGAGATTGAGACTAACATGAATTTAATTGGAGCCACTGCTGTGGAA
GACAAGCTGCAAGATCAGGCTGCAGAGACCATTGAAGCTCTCCATGCAGCTGGCTTAAAAGTCTGGGTGC
TTACTGGGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97019. Forward Primer - name:097019_F_cDNA_Atp11c, sequence:GGATTATCTCAGACTGATGGGC; Reverse Primer - name:097019_N_SP6_cDNA_Atp11c, sequence:CCCCAGTAAGCACCCAGACTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15114 same embryo
 EMAGE:15118 same embryo
 EMAGE:15116 same embryo
 EMAGE:15117 same embryo
 EMAGE:15115 same embryo
 EurExpress:euxassay_010318 same experiment
 MGI:4823302 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS