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EMAGE:15158

Lrrc58 leucine rich repeat containing 58 ( MGI:2443542)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15158 EMAGE:15158 EMAGE:15158 EMAGE:15158 EMAGE:15158
"Pseudo-wholemount" of euxassay_010350. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010350_01 euxassay_010350_02 euxassay_010350_03 euxassay_010350_04
EMAGE:15158 EMAGE:15158 EMAGE:15158 EMAGE:15158 EMAGE:15158
euxassay_010350_05 euxassay_010350_06 euxassay_010350_07 euxassay_010350_08 euxassay_010350_09
EMAGE:15158 EMAGE:15158 EMAGE:15158 EMAGE:15158 EMAGE:15158
euxassay_010350_10 euxassay_010350_11 euxassay_010350_12 euxassay_010350_13 euxassay_010350_14
EMAGE:15158 EMAGE:15158 EMAGE:15158 EMAGE:15158 EMAGE:15158
euxassay_010350_15 euxassay_010350_16 euxassay_010350_17 euxassay_010350_18 euxassay_010350_19
EMAGE:15158 EMAGE:15158 EMAGE:15158 EMAGE:15158
euxassay_010350_20 euxassay_010350_21 euxassay_010350_22 euxassay_010350_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15158Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15158_wholemount_strong.wlz
15158_wholemount_moderate.wlz
15158_wholemount_weak.wlz
15158_wholemount_possible.wlz
15158_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15158_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37604
Entity Detected:Lrrc58, leucine rich repeat containing 58 ( MGI:2443542)
Sequence:sense strand is shown

>T37604
TAACGTATGACCCTCCGACTCTTCTGGAATTAGCTGCAAGGACCATTAAGATCCGAAGTATTTCTTACAC
TCCCTATGACCTTCCTGGGAATCTTCTTAGATACTTGGGTTCAGCCAGCAACGGTCCAAACCCAAAGTGT
GGTGGAGTCTATTTTGACTGTTGTGTCAGACAAATTAAGTTTGTGGACTTCTGTGGGAAGTACCGTCTCC
CCCTCATGCACTATCTGTGCTCTCCAGAGTGCTCCTCCCCCTGCAGCTCTGCCTCCCATAGCTCCACCTC
CCAGAGTGAATCTGACTCAGAAGATGAAGCCAGTGTTGCTGCACACAGAATGCAGAAAGTCCTTCTTGGT
TGAGCAGCAATGGGTATTGAAAAATATTAAAACACTGAACAGAAATGACTAAAAGGAAATGGGTCTTGAA
GTTGATGGGAGACCTGAGCGTTTTCATCTTGAGTGTCCATGTAAGACCTGCCATGATGTGAAAGAAAGGT
TTGGCATTCTGTCTACACATTCGGCAGGAATGAGTCCAGTTCCCTGTTTAGATTCTTTGAGTGTAATTTA
TGGTGAATGGCAGTTCAAAATTCAGTGGATCGGTCTTAATTTCCATGTAAGTCTTTGAAGAAACACTAAC
AGGATGGTTCATTCTGAAGAACAGGCTTGCCTGTAATCAGCCGGAAGTGGGATCGCCTGGGACAGCTGAG
TTGCATAAAGACATCATCACAGTTCTGGTACAAGGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 88571. Forward Primer - name:088571_F_cDNA_C330018J07Rik, sequence:TAACGTATGACCCTCCGACTCT; Reverse Primer - name:088571_N_SP6_cDNA_C330018J07Rik, sequence:CCCTTGTACCAGAACTGTGATG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15157 same embryo
 EMAGE:15160 same embryo
 EMAGE:15161 same embryo
 EMAGE:15156 same embryo
 EMAGE:15159 same embryo
 EurExpress:euxassay_010350 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS