EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:15161

Fkbp15 FK506 binding protein 15 ( MGI:2444782)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010344. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010344_01	euxassay_010344_02	euxassay_010344_03	euxassay_010344_04

euxassay_010344_05	euxassay_010344_06	euxassay_010344_07	euxassay_010344_08	euxassay_010344_09

euxassay_010344_10	euxassay_010344_11	euxassay_010344_12	euxassay_010344_13	euxassay_010344_14

euxassay_010344_15	euxassay_010344_16	euxassay_010344_17	euxassay_010344_18	euxassay_010344_19

euxassay_010344_20	euxassay_010344_21	euxassay_010344_22	euxassay_010344_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15161_wholemount_strong.wlz

15161_wholemount_moderate.wlz

15161_wholemount_weak.wlz

15161_wholemount_possible.wlz

15161_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15161_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 17 18

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 16 17 18 19

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06

spinal cord

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13

dorsal root ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 13 14

neural retina

moderate

regional

moderate expression: see section 02 03 04 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37609
Entity Detected:	Fkbp15, FK506 binding protein 15 ( MGI:2444782)
Sequence:	sense strand is shown >T37609 CTCTCCTTCAGGATGAGTGCTTAGCTCTCCAGGCCCAGATCGCAGCCTTCACTGAACAGAAGGAACACAT GCAGCGACTGGAGAAGACTAAGTCCCAGGCACCTGCAGGCAGAGCTGCTGCTGACCCCTCAGAGAAGGTC AAGAAGATCATGAACCAGGTGTTCCAGTCACTGAGGGGAGAGTTTGAGCTGGAGGAATCTTATGATGGAG GGACCATTCTGAGGACCATCATGCATACAATTAAGATGGTGACGCTGCAGCTGTTGAACCATCAGGAGGA AGAGGAGGAAGAGGAAGAGGAGGAGGAAGAGGAGAAGAAGCCCCTGAGACCTTCCCTGGAGCAGCCAGGC CCTGCCACCCCGGGGATGCCTCCAGCACCCCCAAGTGGGGAGACGCAGGAGGCTCCCGAGGTGCTGCCAG AACAGGTAGTAGGGGAGACCACCCCACTGCCTCTACAGGCTCTCCCCACGCCAGAGAATGGTGCACAGAC AAGGAAAGGGGAGCCCGCAGAAGCGGAGGTACCTTCAGAAATCAAAGACAGTTCTCTCCCACCTCAGCCA GCTGGCATCCCAGCTCACAGAGTCCTGGGGCCCCCGACTTCAATCCCACCTAAACCTCCAGGACCTGTAA CTATGGACTCTGAGTCTGAGGAGATGCTTGCTGCTGACCAGAGAACAGTGCAGCCCAATGGCTTGTTGGG AGAAGAACATGTCAGGGAAGTAGCCACAGATGGTCTACTGCAAGGCAACTCCAGGAGATTGTCACTGACT CCAGACCCTGAGAAGGGAGAGCCACCAGCCTTAGACCCTGAAAGCCAAGGAGGAGAAGCTCAGCCTCCTG AGTGCAAACAAGCTGAGGATGTTAGTAGCTCTGGTCCCCGTGAGACGCTGTTAGACACAGAGCTTGCTTC AGCAGCTGCAGGGACATCCCTCAGGCACAACCAGGACTCCCAGCACTGCAGTCTCTCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 77972. Forward Primer - name:077972_F_cDNA_C430014M02Rik, sequence:CTCTCCTTCAGGATGAGTGCTT; Reverse Primer - name:077972_N_SP6_cDNA_C430014M02Rik, sequence:AGAGAGACTGCAGTGCTGGGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15157 same embryo
	EMAGE:15160 same embryo
	EMAGE:15156 same embryo
	EMAGE:15159 same embryo
	EMAGE:15158 same embryo
	EurExpress:euxassay_010344 same experiment
	MGI:4824870 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source