EMAGE mouse gene expression spatial database

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EMAGE:15164

Hopx HOP homeobox ( MGI:1916782)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010529. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010529_01	euxassay_010529_02	euxassay_010529_03	euxassay_010529_04

euxassay_010529_05	euxassay_010529_06	euxassay_010529_07	euxassay_010529_08	euxassay_010529_09

euxassay_010529_10	euxassay_010529_11	euxassay_010529_12	euxassay_010529_13	euxassay_010529_14

euxassay_010529_15	euxassay_010529_16	euxassay_010529_17	euxassay_010529_18	euxassay_010529_19

euxassay_010529_20	euxassay_010529_21	euxassay_010529_22	euxassay_010529_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15164_wholemount_strong.wlz

15164_wholemount_moderate.wlz

15164_wholemount_weak.wlz

15164_wholemount_possible.wlz

15164_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15164_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

strong

regional

strong expression: see section 05 06 07 20 21

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 13 14

telencephalon ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13 14 16 17 18 weak expression: see section 19 20

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 08 09 11 14 15 moderate expression: see section 12

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 07 08 09 10 13 14 15 16 moderate expression: see section 12

pons mantle layer

strong

regional

strong expression: see section 06

pons ventricular layer

strong

regional

strong expression: see section 06 07 08 09 16 17 18 19 moderate expression: see section 11

midbrain ventricular layer

moderate

regional

moderate expression: see section 13 14 15

spinal cord lateral wall

strong

regional

strong expression: see section 10 11 12

anterior naris

strong

regional

strong expression: see section 11 moderate expression: see section 12 15

external naris

strong

regional

strong expression: see section 16

heart atrium

moderate

regional

moderate expression: see section 05 06 08 09 14 15 16 17 18 19 20

heart ventricle

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

midgut

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30272
Entity Detected:	Hopx, HOP homeobox ( MGI:1916782)
Sequence:	sense strand is shown >T30272 ATCTCCGGAGGCAGCACTTGAGGCGCTTCCTCAGTATACTGTCCCCTCGGAGTGTCAGGCGGGAGGCGTC TTCTTCCTCCTCTCCATCCTTAGTCAGACGCGCACGGACCATGTCGGCGCAGACCGCGAGCGGCCCCACG GAGGACCAGGTGGAGATCCTGGAGTACAACTTCAACAAGGTCAACAAGCACCCGGACCCCACCACGCTGT GCCTCATCGCAGCCGAGGCGGGTCTCACGGAGGAGCAGACGCAGAAATGGTTTAAGCAGCGCCTGGCAGA GTGGCGGCGGTCAGAAGGCTTGCCTTCGGAATGCAGATCTGTTACGGACTAGGGAGCCAGGCCCTTGAGC TTGCTCTTGGAACTCCATCTCTTCTTCCTTCCCTCGGCTTAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4989797), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 33989. Forward Primer - name:033989_F_IRAV71-74_F09_Hod, sequence:ATCTCCGGAGGCAGCACT; Reverse Primer - name:033989_R_SP6_IRAV71-74_F09_Hod, sequence:GGTAAGCCGAGGGAAGGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15163 same embryo
	EMAGE:15162 same embryo
	EMAGE:15166 same embryo
	EMAGE:15167 same embryo
	EMAGE:15165 same embryo
	EurExpress:euxassay_010529 same experiment
	MGI:4825409 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source