EMAGE mouse gene expression spatial database

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EMAGE:15175

Map3k15 mitogen-activated protein kinase kinase kinase 15 ( MGI:2448588)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010331. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010331_01	euxassay_010331_02	euxassay_010331_03	euxassay_010331_04

euxassay_010331_05	euxassay_010331_06	euxassay_010331_07	euxassay_010331_08	euxassay_010331_09

euxassay_010331_10	euxassay_010331_11	euxassay_010331_12	euxassay_010331_13	euxassay_010331_14

euxassay_010331_15	euxassay_010331_16	euxassay_010331_17	euxassay_010331_18	euxassay_010331_19

euxassay_010331_20	euxassay_010331_21	euxassay_010331_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15175_wholemount_strong.wlz

15175_wholemount_moderate.wlz

15175_wholemount_weak.wlz

15175_wholemount_possible.wlz

15175_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15175_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

weak

regional

weak expression: see section 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37587
Entity Detected:	Map3k15, mitogen-activated protein kinase kinase kinase 15 ( MGI:2448588)
Sequence:	sense strand is shown >T37587 CTAAAGGTGGACCTGGACTTTGACAGCTCATCCATCAACCAGATTCACCTGATTCTGTTTGGGTTCCAAG ATGCTGTCAATAGAATTTTGAGAAACCACTTAATTAGGCCCCACTGGATGTTTGCAATGGACAACATCAT TCGCAGAGCTGTGCAGGCTGCAGTCACCATTCTCATTCCAGAGCTCCAAGCCCACTTTGAGCCTGCTTCT GAGACTGAAGGGGTAGACAAGGACACAGAAGTAGAAGGGGACTATCCCCTAGTAGACCTCCTCAGCCAAG AAGTGCATGTGACACCTAGAGGCACCAGACCTGGCTCAGTGGCTATCCAGGAGGGCCAGCCCCACCAGCA AGACCCAAGTCTCCAACTGAGCAAGCTCAGGCAAGAGACCAACAGACTTTGGGAACACCTAGTTCAAAAA GAGAGGGAGTACCAGAATCTTCTTCGCCTAATTCTAGACCAGAAAACTCAAGAATTGTATCACCTTCAGT TACAGTACAAATCCAATGGTGGTACAGAGAACCCTCCACCCCCTGATGGACTGGGAACCGACAGAGAGCT TATAGACTGGTTGCAACTACAAGGAGTGGATGCCAATACAATAGAAAAGATTGTTGAAGAGGACTATACA CTTTCTGATATTCTCAATGATATCACTAAGGAAGACCTAAGGTGCCTCCGACTACGGGGTGGTGTCCTCT GTAGGCTCTGGCATGCAGTCTCCCAGCACAGAAGAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 86991. Forward Primer - name:086991_F_cDNA_BC031147, sequence:CTAAAGGTGGACCTGGACTTTG; Reverse Primer - name:086991_N_SP6_cDNA_BC031147, sequence:GTCTTCTGTGCTGGGAGACTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15176 same embryo
	EMAGE:15177 same embryo
	EMAGE:15173 same embryo
	EMAGE:15174 same embryo
	EurExpress:euxassay_010331 same experiment
	MGI:4826081 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source