EMAGE mouse gene expression spatial database

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EMAGE:15176

Bcl2a1a B-cell leukemia/lymphoma 2 related protein A1a ( MGI:102687)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010322. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010322_01	euxassay_010322_02	euxassay_010322_03	euxassay_010322_04

euxassay_010322_05	euxassay_010322_06	euxassay_010322_07	euxassay_010322_08	euxassay_010322_09

euxassay_010322_10	euxassay_010322_11	euxassay_010322_12	euxassay_010322_13	euxassay_010322_14

euxassay_010322_15	euxassay_010322_16	euxassay_010322_17	euxassay_010322_18	euxassay_010322_19

euxassay_010322_20	euxassay_010322_21	euxassay_010322_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15176_wholemount_strong.wlz

15176_wholemount_moderate.wlz

15176_wholemount_weak.wlz

15176_wholemount_possible.wlz

15176_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15176_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

spotted

moderate expression: see section 10 13

heart ventricle

moderate

spotted

moderate expression: see section 09

mandible

moderate

single cell

moderate expression: see section 03 04 05 07 08 09 16 18 19 20 weak expression: see section 06 14 15 17

maxilla

moderate

single cell

moderate expression: see section 04 05 07 08 09 16 19 20 weak expression: see section 06

clavicle

moderate

single cell

moderate expression: see section 07 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37591
Entity Detected:	Bcl2a1a, B-cell leukemia/lymphoma 2 related protein A1a ( MGI:102687)
Sequence:	sense strand is shown >T37591 ATTAGGGTTCCTCCCCTTACAAGACCCATCTTTGCAGTCTTTGCCTCCTTGGCCTCTAGGTGGACAGCTG CAGTCAGCTCTGCCAGGGAAGATGGCTGAGTCTGAGCTCATGCATATCCACTCCCTGGCTGAGCACTACC TTCAGTATGTGCTACAGGTACCCGCCTTTGAGTCGGCTCCAAGCCAAGCATGCAGAGTGCTACAAAGAGT TGCTTTCTCCGTTCAGAAGGAAGTTGAAAAGAATCTGAAGTCATACTTGGATGACTTTCACGTGGAATCC ATAGATACCGCCAGAATAATATTCAACCAAGTGATGGAAAAAGAGTTTGAAGATGGCATCATTAACTGGG GAAGGATTGTGACTATATTTGCCTTTGGGGGTGTTCTCCTCAAAAAACTTCCACAAGAGCAGATTGCCCT GGATGTATGTGCTTACAAACAAGTTTCCAGTTTTGTGGCAGAATTCATAATGAATAACACAGGAGAATGG ATACGGCAGAATGGAGGTTGGGAAGATGGCTTCATAAAGAAGTTTGAACCCAAATCTGGCTGGCTGACTT TTCTGCAGATGACAGGACAGATCTGGGAAATGCTCTTTCTCCTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 95259. Forward Primer - name:095259_F_cDNA_Bcl2a1a, sequence:ATTAGGGTTCCTCCCCTTACAA; Reverse Primer - name:095259_N_SP6_cDNA_Bcl2a1a, sequence:GAGGAGAAAGAGCATTTCCCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15177 same embryo
	EMAGE:15175 same embryo
	EMAGE:15173 same embryo
	EMAGE:15174 same embryo
	EurExpress:euxassay_010322 same experiment
	MGI:4823445 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source