EMAGE mouse gene expression spatial database

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EMAGE:15190

Gng3 guanine nucleotide binding protein (G protein), gamma 3 ( MGI:102704)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010359. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010359_01	euxassay_010359_02	euxassay_010359_03	euxassay_010359_04

euxassay_010359_05	euxassay_010359_06	euxassay_010359_07	euxassay_010359_08	euxassay_010359_09

euxassay_010359_10	euxassay_010359_11	euxassay_010359_12	euxassay_010359_13	euxassay_010359_14

euxassay_010359_15	euxassay_010359_16	euxassay_010359_17	euxassay_010359_18	euxassay_010359_19

euxassay_010359_20	euxassay_010359_21	euxassay_010359_22	euxassay_010359_23	euxassay_010359_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15190_wholemount_strong.wlz

15190_wholemount_moderate.wlz

15190_wholemount_weak.wlz

15190_wholemount_possible.wlz

15190_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15190_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 moderate expression: see section 01 02 03 04 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 07 19 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 08 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 17 18 19

trigeminal v nerve

strong

regional

strong expression: see section 10 17

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 15

cervical ganglion

strong

regional

strong expression: see section 08 09 16 17

thoracic ganglion

strong

regional

strong expression: see section 10 11 12 13 14

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

neural retina

strong

regional

strong expression: see section 02 03 04 05 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 16 17 weak expression: see section 11 12 13 14 15 18 19 20

tongue

moderate

spotted

moderate expression: see section 13 16

stomach

weak

spotted

weak expression: see section 04 05 06 07 08 09 10 11

midgut

moderate

spotted

moderate expression: see section 12 13 weak expression: see section 11 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30250
Entity Detected:	Gng3, guanine nucleotide binding protein (G protein), gamma 3 ( MGI:102704)
Sequence:	sense strand is shown >T30250 CTGAGGCAGGGTTTGGTGCCCCCTCCCCACTGACCCTACATCCCTGGCTGCCGCTGCTGTTACTTCAGGA TGAAAGGGGAGACCCCCGTTAACAGCACTATGAGTATTGGTCAAGCACGCAAGATGGTGGAACAGCTTAA GATTGAGGCCAGCTTGTGCCGGATAAAGGTGTCCAAGGCAGCAGCAGACCTGATGACATACTGTGATGCC CACGCTTGTGAGGACCCCCTCATCACTCCTGTGCCCACTTCCGAGAACCCCTTCCGGGAGAAGAAGTTCT TCTGTGCGCTCCTCTGAAGAGCGGTGGACCTCCTCTGAACTCATCGCCCCCTCCCAGACCCGCCCTTAGA TTGGAAATTGTAGTAGATCACTTCTACCCGCATTTACCCGGAGATTATTTGAAGCACAAGGCCCTCCTTA CCCATGTCTACCCCATCTCCTCACCCTTTCAGGCTAACTCCAAGCACCTTCTACTCTATCTATAAAGGGA CCCCGCTAAACTGCCAAAATCCTGCCTGTTTCCCTCGGTGATCCATCCGTTCAGGCAATGGAGAGGGGGA TGCCCGGGTGCTCACCTGGAGCTATGAAAGGTAAAGGCATTTGAAGAATAAAGTCATTCAGAGCCTCAGA GG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5365311), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 9700. Forward Primer - name:ABA_MGC_015_02_H02_F_AAGGAGCAACAGCGATGG_ABA_REV_01, sequence:CTGAGGCAGGGTTTGGTG; Reverse Primer - name:_R_SP6_ABA_MGC_011_02_D07_CTGAGGCAGGGTTTGGTGCCCCCT, sequence:TCCTCTGAGGCTCTGAATGAC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15191 same embryo
	EMAGE:15192 same embryo
	EurExpress:euxassay_010359 same experiment
	MGI:4825138 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source