EMAGE mouse gene expression spatial database

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EMAGE:15234

Erp27 endoplasmic reticulum protein 27 ( MGI:1916437)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010436. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010436_01	euxassay_010436_02	euxassay_010436_03	euxassay_010436_04

euxassay_010436_05	euxassay_010436_06	euxassay_010436_07	euxassay_010436_08	euxassay_010436_09

euxassay_010436_10	euxassay_010436_11	euxassay_010436_12	euxassay_010436_13	euxassay_010436_14

euxassay_010436_15	euxassay_010436_16	euxassay_010436_17	euxassay_010436_18	euxassay_010436_19

euxassay_010436_20	euxassay_010436_21	euxassay_010436_22	euxassay_010436_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15234_wholemount_strong.wlz

15234_wholemount_moderate.wlz

15234_wholemount_weak.wlz

15234_wholemount_possible.wlz

15234_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15234_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 13 14 15 moderate expression: see section 16

midbrain mantle layer

strong

regional

strong expression: see section 04 05 06 07 08 09 13 moderate expression: see section 03 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37292
Entity Detected:	Erp27, endoplasmic reticulum protein 27 ( MGI:1916437)
Sequence:	sense strand is shown >T37292 TAATCCTCTCGTTTGTCCTCGTGTGTGGGCTGGTTCCAGAGGTTACAGCAGATGTGGAAGAAGCCACGGA TGGTCTCAGCACTACCCAGGAACCCATATGGCTCACAGACGTCCCAGCCACCGTGGAACTCATCGCTGCT GCTGAAGTGGCAGTCATAGGCTTCTTCCAGGATTTAGAAATACCGATCGTGTCTGTGTTCCGGAGCATGG CACGACAGTTCCAAGATGTATCGTTTGGAATCAGCAACCATTCTGAGGTCCTGACCCATTACAATGTCAC CAGCAACAGCATCTGCCTCTTTCGACTGGTTGATGATCAACAACTACATTTGAATGCTGAAGACATTGAA AATCTCGATGCTGCCAAATTGAGCCGTTTTATTCACGTGAACAACCTCCACTGGGTGACAGAGTACAGTC CTATGATTGCAGCTGGCTTATTTAACACCATGGTTCAGACCCATCTTCTCCTGATGATGAAGAAGACTTC CCCAGAGTATGAAGAGAGCATGCGCAGATATCGGGAGGCAGCTAAGCTCTTCCAGGGACAGATTCTCTTT GTTCTGGTGGACAGCGGCAAAAGGGAAAATGGAAAGGTGATGTCCTATTTCAAACTAAAAGAGTCTCAGC TGCCAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 65220. Forward Primer - name:065220_F_cDNA_1810033M07Rik, sequence:TAATCCTCTCGTTTGTCCTCGT; Reverse Primer - name:065220_N_SP6_cDNA_1810033M07Rik, sequence:CTGGCAGCTGAGACTCTTTTAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15232 same embryo
	EMAGE:15235 same embryo
	EMAGE:15231 same embryo
	EMAGE:15233 same embryo
	EurExpress:euxassay_010436 same experiment
	MGI:4824617 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source