EMAGE mouse gene expression spatial database

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EMAGE:15250

Copg coatomer protein complex, subunit gamma ( MGI:1858696)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010470. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010470_01	euxassay_010470_02	euxassay_010470_03	euxassay_010470_04

euxassay_010470_05	euxassay_010470_06	euxassay_010470_07	euxassay_010470_08	euxassay_010470_09

euxassay_010470_10	euxassay_010470_11	euxassay_010470_12	euxassay_010470_13	euxassay_010470_14

euxassay_010470_15	euxassay_010470_16	euxassay_010470_17	euxassay_010470_18	euxassay_010470_19

euxassay_010470_20	euxassay_010470_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15250_wholemount_strong.wlz

15250_wholemount_moderate.wlz

15250_wholemount_weak.wlz

15250_wholemount_possible.wlz

15250_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15250_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 08 14 15 16 17 18 19

mandible

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 13 14 15 16 17 18 19

maxilla

moderate

regional

moderate expression: see section 05 06 07 08 15 16 17 18 19

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 05 06 07 19 20 21

viscerocranium

moderate

regional

Expression in the turbinate bone.

clavicle

moderate

regional

moderate expression: see section 05 06 13 weak expression: see section 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30221
Entity Detected:	Copg, coatomer protein complex, subunit gamma ( MGI:1858696)
Sequence:	sense strand is shown >T30221 CAGCCGGAGAAGGTAGCAGCCACACGGCAGGAGATTTTCCAAGAGCAGCTAGCAGCGGTGCCTGAGTTCC AGGGACTAGGGCCTCTCTTCAAGTCCTCTCCTGAGCCAGTGGCCCTCACAGAGTCCGAGACCGAGTATGT CATCCGTTGCACCAAACACACCTTCTCTGATCACTTGGTGTTCCAGTTTGACTGCACAAACACCCTCAAT GACCAGACTCTGGAGAATGTCACAGTGCAGATGGAGCCCACTGAGGCATACGAAGTGCTCTCGTATGTGC CTGCGCGGAGCTTGCCCTACAACCAGCCTGGGACCTGCTACACACTAGTGGCTCTGCCCACTGAAGACCC CACAGCTGTGGCATGCACGTTCAGCTGTGTGATGAAGTTCACTGTTAAGGACTGTGATCCCAACACAGGA GAAATCGATGAAGAAGGCTATGAGGATGAGTATGTGCTGGAGGATCTGGAAGTTACTGTGGCTGATCACA TCCAAAAAGTCATGAAAGTGAACTTTGAGGCAGCCTGGGATGAGGTTGGGGATGAATTTGAGAAGGAGGA AACGTTCACCCTGTCTACTATCAAGACACTCGAAGAGGCTGTGGGCAATATTGTGAAGTTCCTGGGAATG CCTCCTTGTGAGAGGTCAGACAAAGTGCCAGAAAACAAGAACACCCACACGCTGCTGCTAGCTGGAGTAT TCCGGGGTGGTCATGACATCCTTGTGCGTTCTCGGCTTCTGCTTTTGGACACAGTGACGATGCAGGTGAC AGCCAGAAGCTCAGAGGAGCTGCCAGTAGATATCATCTTGGCATCCGTGGGCTAGAGGTGGCCTAAGCCC CACTCCTATCTTGTCCAACACTACGTCGAAGCCACTTCTTCCCTGTGAAGCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4189311), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 9469. Forward Primer - name:009469_F_IRAV55-58_E17_Copg, sequence:CAGCCGGAGAAGGTAGCA; Reverse Primer - name:009469_R_SP6_IRAV55-58_E17_Copg, sequence:CGGCTTCACAGGGAAGAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15247 same embryo
	EMAGE:15249 same embryo
	EMAGE:15251 same embryo
	EMAGE:15248 same embryo
	EurExpress:euxassay_010470 same experiment
	MGI:4824005 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source