EMAGE mouse gene expression spatial database

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EMAGE:15280

Mlc1 megalencephalic leukoencephalopathy with subcortical cysts 1 homolog (human) ( MGI:2157910)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010374. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010374_01	euxassay_010374_02	euxassay_010374_03	euxassay_010374_04

euxassay_010374_05	euxassay_010374_06	euxassay_010374_07	euxassay_010374_08	euxassay_010374_09

euxassay_010374_10	euxassay_010374_11	euxassay_010374_12	euxassay_010374_13	euxassay_010374_14

euxassay_010374_15	euxassay_010374_16	euxassay_010374_17	euxassay_010374_18	euxassay_010374_19

euxassay_010374_20	euxassay_010374_21	euxassay_010374_22	euxassay_010374_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15280_wholemount_strong.wlz

15280_wholemount_moderate.wlz

15280_wholemount_weak.wlz

15280_wholemount_possible.wlz

15280_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15280_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 13 14

telencephalon ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata alar plate ventricular layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 02 03 05 06 07 08 09 10 15 16 weak expression: see section 04 11 12 13 14

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 05 06 07 09 18 weak expression: see section 08

pons ventricular layer

moderate

regional

moderate expression: see section 03 05 06 07 09 10 15 16 17 18 weak expression: see section 04 08 11 12 13 14

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13

spinal cord ventricular layer

moderate

regional

moderate expression: see section 06 07 weak expression: see section 08 09 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30254
Entity Detected:	Mlc1, megalencephalic leukoencephalopathy with subcortical cysts 1 homolog (human) ( MGI:2157910)
Sequence:	sense strand is shown >T30254 AAAGATTACCCGCCAGCCATCAAATCTTATGATGTTCTCCTGCTGCTCCTGTTGCTGCTGCTGCTGCTTC AGGGGGGACTCAACACTGGCACCGCCATCCAGTGTGTGAGCTTCAAAGTCAGCGCCCGGCTGCAGGCTGC ATCCTGGGACCCCCAGTCCTGTCCCCAGGAGCGGCCAGCAGGGGAGGTGGTCCGAGGCCCCCTCAAGGAG TTTGACAAAGAGAAAGCCTGGAGAGCTGTGGTGGTACAAATGGCCCAGTGACTACAGGATAAAGAGACTG AGTCTGGGTGTCTGCCCGGACCCCAGCGTGCTCCACCGTACGGACTCTAACCATTGCACGGCTACTGCTT TCTAACATACTATTTCCCTCCTGTGAGGGCAGCCCAGGCTGCAAGGTGGCTTTTCGGTTCCTGTCGTAGT TTAACTTTGAAACAGGAAAATATTGAGTGTTGGGCCCTATTAGGTCTCAGTGTGTGCACCTCAGGTGACC CTGATGGCCTGGGTGTTGCTTTTTTTTCCAGCCCTCCTCACTTCCTGGGTTGACCCTCATTCACCAGGAC CTGGTGTTCTGGCCTTAGGGCACTTCCATGCCTTCTCATGCTTGCCACAGTGCAGGGATAGTTTGGCTGT GAGACTCACAGATAGTTTGTATATCACCTCCCCAGGCCCCAGAAGACAGCTGGACTCTCCCAGCTAACCA GAGAATCAAGGAACCTATAGTAAGTTGGGTCCTTTGTGAAGCTGGGGATCATTTTAGGAATTGCTTTCTG GGAGGGGAGCCTCTGTGCTTGGGAAGACAGCGAGCGTGCCTTTACTGAGCTGGGGTATCAATGGGTTCCC TTCCAGGAAGAGGTATATTCTGTGCCTCAGAAGAGCTGCCAGGGTCAGGGAGAGAGTGAAAATCCTTCTA GGCAGCTCACTTGTAGATACACACGACTGCCAGGATTCAGGAAAAGCGTGGCCATTTTCAAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5363238), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 32319. Forward Primer - name:032319_F_IRAV63-66_P15_Mlc1, sequence:AAAGATTACCCGCCAGCC; Reverse Primer - name:032319_R_SP6_IRAV63-66_P15_Mlc1, sequence:GTTTGAAAATGGCCACGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15279 same embryo
	EMAGE:15277 same embryo
	EMAGE:15276 same embryo
	EMAGE:15278 same embryo
	EurExpress:euxassay_010374 same experiment
	MGI:4826389 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source