EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:15301

Fgf15 fibroblast growth factor 15 ( MGI:1096383)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010475. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010475_01	euxassay_010475_02	euxassay_010475_03	euxassay_010475_04

euxassay_010475_05	euxassay_010475_06	euxassay_010475_07	euxassay_010475_08	euxassay_010475_09

euxassay_010475_10	euxassay_010475_11	euxassay_010475_12	euxassay_010475_13	euxassay_010475_14

euxassay_010475_15	euxassay_010475_16	euxassay_010475_17	euxassay_010475_18	euxassay_010475_19

euxassay_010475_20	euxassay_010475_21	euxassay_010475_22	euxassay_010475_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15301_wholemount_strong.wlz

15301_wholemount_moderate.wlz

15301_wholemount_weak.wlz

15301_wholemount_possible.wlz

15301_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15301_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

strong

regional

strong expression: see section 11 14

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 12 13 14

telencephalon mantle layer

strong

regional

strong expression: see section 09 10 11 12 14 15 16 moderate expression: see section 17 weak expression: see section 08 18

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 10 11 15 16

telencephalon ventricular layer

strong

regional

strong expression: see section 02 03 04 05 10 11 12 14 20 21 22 moderate expression: see section 13 19 weak expression: see section 06

medulla oblongata alar plate ventricular layer

moderate

regional

moderate expression: see section 06 07 08 15 16 17

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 09 10 13 14 moderate expression: see section 08 15

rest of cerebellum mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 14 15 16 17 18 19 20 moderate expression: see section 08 09 10 13

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 14 15 16 17 18 19 20 moderate expression: see section 09 10 11 13

pons ventricular layer

moderate

regional

moderate expression: see section 06 07 15 16 17

midbrain ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 moderate expression: see section 07 08 15 weak expression: see section 16

dorsal grey horn

strong

single cell

strong expression: see section 10 11

retina nuclear layer

strong

homogeneous

strong expression: see section 01 02 03 04 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 14 15 16 weak expression: see section 08 09 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30218
Entity Detected:	Fgf15, fibroblast growth factor 15 ( MGI:1096383)
Sequence:	sense strand is shown >T30218 GCCCAGAGAACAGCTCCAGGACCAGAAACCCTCAAACTTTATCCCCGTGTTTCACCGCTCCTTCTTTGAA ACCGGGGACCAGCTGAGGTCTAAAATGTTCTCCCTGCCCCTGGAGAGTGACAGCATGGATCCGTTCAGGA TGGTGGAGGATGTAGACCACCTAGTGAAGAGTCCCAGCTTCCAGAAATGACAGGATTCCGACAGGATGGA GAAAACCCCAAGGTCCCGTGAACTTCCCCCTTAGGAAGCTGTACATATTCTAAGTCTCACATGGACCCTG TTGTGTTAGTGGCTAGACTTGATCATGAACCTAAATTGACAACCTGCCTGGCTGCCATCGGAGCCCCACT GACTTTGGAGGCTGCTGATATGTGCCTAAGTTACTCCAGTTCTGTTTGAATACCTCCACTAATAGGGAAC TTACTCCTGTGAAACATTCTTAGTTTTGAGCCAAATCTGTGACTTGGATGGTTTTAGCGAGGAAGCCAGA AGGTATGAAGTCAAATGATAAAATTCATGTATAGAAAGTGGGCTCTAAAATATATATTCCCTATATGGAT CTCATGGGATCTTAGCTTGCCCCCCAAATGTCTCCTGGCCAGAACTAACTGGGGTTACAAAACTTGGAAC AAAGGACAGCCTAGAAAACTTTGGGAGCCTTGAAGGATGGTCTTAGGATTACGAATTCCAGCTGACTACG TAGCTTCCCCCTTTTCCACTTATAAATGTCAGATGGAAGTGACCCTTAGCTGAGTGCATAGCCAAGCTGC CACTTAGGCCCCAGGAGCTTGTCTCTGTCCCATGACCCCAGATTTCCAGGACCTGGATCTTCTCCTCTGA CCTTTCCCAGAGTTCACCTGGGCTCTCCAACCCCAGAGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5066286), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 24072. Forward Primer - name:024072_F_IRAV55-58_B22_Fgf15, sequence:GCCCAGAGAACAGCTCCA; Reverse Primer - name:024072_R_SP6_IRAV55-58_B22_Fgf15, sequence:TGCTCTGGGGTTGGAGAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15299 same embryo
	EMAGE:15302 same embryo
	EMAGE:15304 same embryo
	EMAGE:15300 same embryo
	EMAGE:15303 same embryo
	EurExpress:euxassay_010475 same experiment
	MGI:4824841 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source