EMAGE mouse gene expression spatial database

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EMAGE:15325

Mmp11 matrix metallopeptidase 11 ( MGI:97008)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010503. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010503_01	euxassay_010503_02	euxassay_010503_03	euxassay_010503_04

euxassay_010503_05	euxassay_010503_06	euxassay_010503_07	euxassay_010503_08	euxassay_010503_09

euxassay_010503_10	euxassay_010503_11	euxassay_010503_12	euxassay_010503_13	euxassay_010503_14

euxassay_010503_15	euxassay_010503_16	euxassay_010503_17	euxassay_010503_18	euxassay_010503_19

euxassay_010503_20	euxassay_010503_21	euxassay_010503_22	euxassay_010503_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15325_wholemount_strong.wlz

15325_wholemount_moderate.wlz

15325_wholemount_weak.wlz

15325_wholemount_possible.wlz

15325_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15325_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 18 19 20 21 22 23

medulla oblongata floor plate

strong

regional

strong expression: see section 13

spinal cord floor plate

strong

regional

strong expression: see section 08 09 10 11 12 13

inner ear

strong

regional

strong expression: see section 21 moderate expression: see section 02 03 04 05 16 17 18 19 20

pharynx epithelium

moderate

regional

moderate expression: see section 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30500
Entity Detected:	Mmp11, matrix metallopeptidase 11 ( MGI:97008)
Sequence:	sense strand is shown >T30500 TTCCACCATCCGAGGAGAGCTCTTCTTCTTCAAGGCAGGCTTTGTGTGGAGGCTGCGCAGTGGGCGACTG CAGCCCGGGTATCCTGCTTTGGCCTCTCGGCACTGGCAAGGACTGCCCAGCCCTGTGGATGCAGCTTTTG AGGATGCCCAGGGCCAGATTTGGTTCTTCCAAGGTGCTCAGTACTGGGTATATGATGGTGAGAAGCCAGT CCTAGGCCCTGCACCACTCTCCAAGCTGGGCCTGCAAGGGTCCCCAGTTCATGCCGCCTTGGTCTGGGGT CCTGAGAAGAACAAGATCTACTTCTTCCGAGGTGGAGACTATTGGCGTTTCCACCCCAGAACCCAGCGAG TGGACAATCCCGTGCCCCGGCGCTCCACTGACTGGCGAGGGGTACCTTCTGAGATTGATGCTGCCTTCCA GGATGCTGAGGGCTATGCCTACTTCCTTCGTGGCCATCTCTACTGGAAGTTTGATCCCGTGAAGGTGAAG GTCCTAGAAGGCTTTCCTCGCCCCGTAGGTCCTGACTTCTTTGACTGTGCTGAGCCTGCCAATACTTTCC GCTGACAACACTTTGGATGCATTCAGGGGTACTGACTCCTGCCAGGGCACTTAGATCATGTAAGAGACCC ACAGCCATATCTGTGGCTCTGGGCTTCAGGCATGGGACAGACAGGGCCTATGTCTCCTCAGGGGAGTGGG TTGGGGTGCAGCCACTGTTTGTAGGAACGACCATGCTGGTCATGGTCACCTGCCAACAATTGTCTCAGAC TAGGCAAAGGCTTTGGTGTTACTTAAAAATAAGGGAGGTTTTGGGCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:30058638), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58818. Forward Primer - name:058818_F_IRAV108_e12_Mmp11, sequence:TTCCACCATCCGAGGAGA; Reverse Primer - name:058818_R_SP6_IRAV108_e12_Mmp11, sequence:CAGCCCAAAACCTCCCTT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15324 same embryo
	EMAGE:15327 same embryo
	EMAGE:15326 same embryo
	EMAGE:15323 same embryo
	EMAGE:15328 same embryo
	EurExpress:euxassay_010503 same experiment
	MGI:4826403 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source