EMAGE mouse gene expression spatial database

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EMAGE:15332

Lcmt1 leucine carboxyl methyltransferase 1 ( MGI:1353593)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010513. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010513_01	euxassay_010513_02	euxassay_010513_03	euxassay_010513_04

euxassay_010513_05	euxassay_010513_06	euxassay_010513_07	euxassay_010513_08	euxassay_010513_09

euxassay_010513_10	euxassay_010513_11	euxassay_010513_12	euxassay_010513_13	euxassay_010513_14

euxassay_010513_15	euxassay_010513_16	euxassay_010513_17	euxassay_010513_18	euxassay_010513_19

euxassay_010513_20	euxassay_010513_21	euxassay_010513_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15332_wholemount_strong.wlz

15332_wholemount_moderate.wlz

15332_wholemount_weak.wlz

15332_wholemount_possible.wlz

15332_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15332_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex mantle layer

moderate

regional

moderate expression: see section 10 11 12 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30789
Entity Detected:	Lcmt1, leucine carboxyl methyltransferase 1 ( MGI:1353593)
Sequence:	sense strand is shown >T30789 ACCATCAAAAGCAAGCCGCTTTTATTCAGACCCATCATGGAGTTACACCCAGAGGACACTCTTCAAATGG ATAGTCACATGCTGGATTCAAAAAGATATGCCATCATTGGAGCAGATCTCCGAGACCTTTCTGAGCTGGA AGAGAAACTAAAGAAATGTAACATGAATACACAATTGCCAACACTCCTGATAACTGAATGTGTGCTGGTT TACATGACTCCAGAGCAGTCAGCCAACCTCCTGAAATGGGCAGCCAGCAGTTTTGAGACAGCCATGTTCA TAAACTATGAGCAGGTCAACATGGACGACCGGTTTGGGCAGATCATGATTGAAAACCTCCGGAGGCGAAG TTGTGACCTGGCAGGAGTGGAAACCTGCAAATCACTGGAGTCCCAGAAGGAACGGCTGCTGTTGAATGGG TGGGAGACAGCGTCTGCAGTGAACATGATGGAGCTGTACAGTGGGCTACCTCGGGCAGAGGTAAACAGAA TAGAATCCCTCGAGTTCCTGGATGAGATGGAGCTCCTGGAGCAGCTCATGCGCCATTACTGCCTGTGCTG GGCCACCCGCGGTGGCCAGGAGCTGGGTTTGAAGGAGATAACCTATTGATCTGCTGAAGGCTCGTGCTGG GGTGGAAGGTGAAGGCTCTAGCTAAGGGAGTGCCAGGGCCTCCTGAGAGATGTGCAGCCTCACTTTGTTG GTCCTGCTGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3595687), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 16045. Forward Primer - name:016045_F_IRAV16-19_F14_D4Wsu132e, sequence:ACCATCAAAAGCAAGCCG; Reverse Primer - name:016045_R_SP6_IRAV16-19_F14_D4Wsu132e, sequence:TGCAGCAGGACCAACAAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15330 same embryo
	EMAGE:15331 same embryo
	EMAGE:15329 same embryo
	EMAGE:15333 same embryo
	EurExpress:euxassay_010513 same experiment
	MGI:4825885 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source