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EMAGE:15339

Cby1 chibby homolog 1 (Drosophila) ( MGI:1920989)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15339 EMAGE:15339 EMAGE:15339 EMAGE:15339 EMAGE:15339
"Pseudo-wholemount" of euxassay_010516. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010516_01 euxassay_010516_02 euxassay_010516_03 euxassay_010516_04
EMAGE:15339 EMAGE:15339 EMAGE:15339 EMAGE:15339 EMAGE:15339
euxassay_010516_05 euxassay_010516_06 euxassay_010516_07 euxassay_010516_08 euxassay_010516_09
EMAGE:15339 EMAGE:15339 EMAGE:15339 EMAGE:15339 EMAGE:15339
euxassay_010516_10 euxassay_010516_11 euxassay_010516_12 euxassay_010516_13 euxassay_010516_14
EMAGE:15339 EMAGE:15339 EMAGE:15339 EMAGE:15339 EMAGE:15339
euxassay_010516_15 euxassay_010516_16 euxassay_010516_17 euxassay_010516_18 euxassay_010516_19
EMAGE:15339 EMAGE:15339 EMAGE:15339 EMAGE:15339
euxassay_010516_20 euxassay_010516_21 euxassay_010516_22 euxassay_010516_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15339Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15339_wholemount_strong.wlz
15339_wholemount_moderate.wlz
15339_wholemount_weak.wlz
15339_wholemount_possible.wlz
15339_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15339_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 14 15 16 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30794
Entity Detected:Cby1, chibby homolog 1 (Drosophila) ( MGI:1920989)
Sequence:sense strand is shown

>T30794
TCTCGCTGGGTCAGGAAGGAGAAGGAAGTGCTGGCGTTTCCTCACTGAGTTAGGGATGCCTCTCTTTGGC
AGCATATTCAGCCCAAAGAAGACCCCTCCTCGGAAGTCGGCCTCTCTCTCCAACCTACATTCTTTAGATC
GGTCAACGCGGGAACTGGAGCTGGGGCTCGACTATGGAACTCCTACCATGAACCTGGCGGGGCAGAGCCT
GAAGTTTGAAAACGGCCAGTGGGTGGCAGACTCCGTGATTAGTGGGGGTGTGGACCGGAGGGAAACTCAG
CGCCTGCGCAAACGAAACCAACAGTTAGAAGAAGAAAACAATCTCCTGCGGCTGAAAGTGGACATTCTGC
TGGACATGCTTTCAGAAACCACTGCTGAGTCCCACCTAAAGGACAAAGAGCTGGATGAACTGAAGGTTAC
CAACCGCAGGAGGAAGTGAAGAGAGCCTGGCAGCACTCGCTAGGGGACTGGAAAGAGCCCAGTGACCAGG
GGAGGAGGATGCGGCAGAGCCCTTTGATTTCCCGTCAGATTAGCAGACTAGGGGAGAGCATTAACAGGCC
CTGGCTCCCTTGGGCAGGGGATAGGGAGGAGACACAGGATGGAAGAACCCAAGGTGCCAGGCCAGGGCCA
TAGCCAAGCAAGCACCCCAGGTCCACCCAGGGTGTCAATCTAAAACATACCCCAGGAAGGTCTAGAAATA
ATGAGCCTTGGATGCTGTCGGCAAGTGACAGCATGTAACTGCCTGTGTCTCAGAATCACCATATTCAGAC
AGCTGGCAGCTCACCTCTTGACACTGGTGTCTGGGGTTTGGCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3596486), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 16070. Forward Primer - name:016070_F_IRAV16-19_G16_1110014P06Rik, sequence:TCTCGCTGGGTCAGGAAG; Reverse Primer - name:016070_R_SP6_IRAV16-19_G16_1110014P06Rik, sequence:GTGCCAAACCCCAGACAC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15338 same embryo
 EMAGE:15335 same embryo
 EMAGE:15334 same embryo
 EMAGE:15337 same embryo
 EMAGE:15336 same embryo
 EurExpress:euxassay_010516 same experiment
 MGI:4823659 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS