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EMAGE:15363

H2-T23 histocompatibility 2, T region locus 23 ( MGI:95957)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15363 EMAGE:15363 EMAGE:15363 EMAGE:15363 EMAGE:15363
"Pseudo-wholemount" of euxassay_010562. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010562_01 euxassay_010562_02 euxassay_010562_03 euxassay_010562_04
EMAGE:15363 EMAGE:15363 EMAGE:15363 EMAGE:15363 EMAGE:15363
euxassay_010562_05 euxassay_010562_06 euxassay_010562_07 euxassay_010562_08 euxassay_010562_09
EMAGE:15363 EMAGE:15363 EMAGE:15363 EMAGE:15363 EMAGE:15363
euxassay_010562_10 euxassay_010562_11 euxassay_010562_12 euxassay_010562_13 euxassay_010562_14
EMAGE:15363 EMAGE:15363 EMAGE:15363 EMAGE:15363 EMAGE:15363
euxassay_010562_15 euxassay_010562_16 euxassay_010562_17 euxassay_010562_18 euxassay_010562_19
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euxassay_010562_20 euxassay_010562_21 euxassay_010562_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15363Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15363_wholemount_strong.wlz
15363_wholemount_moderate.wlz
15363_wholemount_weak.wlz
15363_wholemount_possible.wlz
15363_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15363_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30831
Entity Detected:H2-T23, histocompatibility 2, T region locus 23 ( MGI:95957)
Sequence:sense strand is shown

>T30831
TTTTTGCCCACTTGCTCCAGCTGCTGGTCAGCGCCACAGTCCCGACCCAGAGTAGTTCACATTCGCTGCG
GTATTTCCACACTGCCATGTCCCGGCCCGGCCTCGGGGAGCCCCGGTTCATAGTCGTTGGCTACGTGGAC
GACACGCAGTTCGTGAGCTTCGACAGCGACTCGGAGAATCCGAAGATGGAGCCTCGGGCGCGGTGGATGG
AGCAGGAGGGGCCGGAGTATTGGGAGCGGGAGACTTGGAAAGCCAGGGACATGGGGAGGAACTTCAGAGT
AAACCTGAGGACCCTGCTCGGCTACTACAATCAGAGTAAGGACGAATCTCACACGCTGCAGTGGATGTAC
GGCTGCGACGTGGGGCCCGATGGGCGCCTGCTCCGCGGGTATTGTCAGGAGGCCTACGATGGCCAGGATT
ACATCTCCCTGAACGAGGACCTGCGCTCCTGGACCGCGACTGACTTTGCCTCACAGATCTCTAAGCACAA
GTCAGAGATGGTCGATGAGGCCCACCAACAGAGGGCATACCTGCAAGGTCCTTGCGTGGAGTGGCTCCAT
ACATACCTACGGCTGGGAAATAAGACACTGAAGCGCTCAGACCCTCCAAAGGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3709267), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 35082. Forward Primer - name:035082_F_IRAV20-23_E24_H2-T23, sequence:TTTTTGCCCACTTGCTCC; Reverse Primer - name:035082_R_SP6_IRAV20-23_E24_H2-T23, sequence:TGCCTTTGGAGGGTCTGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15362 same embryo
 EMAGE:15361 same embryo
 EMAGE:15364 same embryo
 EurExpress:euxassay_010562 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS