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EMAGE:15372

Ggt1 gamma-glutamyltransferase 1 ( MGI:95706)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15372 EMAGE:15372 EMAGE:15372 EMAGE:15372 EMAGE:15372
"Pseudo-wholemount" of euxassay_010561. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010561_01 euxassay_010561_02 euxassay_010561_03 euxassay_010561_04
EMAGE:15372 EMAGE:15372 EMAGE:15372 EMAGE:15372 EMAGE:15372
euxassay_010561_05 euxassay_010561_06 euxassay_010561_07 euxassay_010561_08 euxassay_010561_09
EMAGE:15372 EMAGE:15372 EMAGE:15372 EMAGE:15372 EMAGE:15372
euxassay_010561_10 euxassay_010561_11 euxassay_010561_12 euxassay_010561_13 euxassay_010561_14
EMAGE:15372 EMAGE:15372 EMAGE:15372 EMAGE:15372 EMAGE:15372
euxassay_010561_15 euxassay_010561_16 euxassay_010561_17 euxassay_010561_18 euxassay_010561_19
EMAGE:15372 EMAGE:15372
euxassay_010561_20 euxassay_010561_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15372Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15372_wholemount_strong.wlz
15372_wholemount_moderate.wlz
15372_wholemount_weak.wlz
15372_wholemount_possible.wlz
15372_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15372_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
metanephros
strong strong
regionalstrong expression: see section 16 18 moderate expression: see section 05 06 07 14 15 19
maturing glomerular tuft
strong strong
regionalstrong expression: see section 04 16 18 19 moderate expression: see section 05 06 07 14 15 weak expression: see section 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30840
Entity Detected:Ggt1, gamma-glutamyltransferase 1 ( MGI:95706)
Sequence:sense strand is shown

>T30840
GGGTTTGGCAATAGCCCTGGACAAAAAACGGGACGTCATTGAGAAGACCCCTGCCTTGTGCGAGGTGTTC
TGCCGCCAAGGGAAGGTGCTTCAAGAAGGAGAGACGGTGACTATGCCGAAGCTGGCTGACACATTGCAGA
TACTGGCCCAGGAGGGCGCCAAGGCCTTCTACAATGGGAGCCTCACAGCCCAGATTGTGAAAGACATCCA
GGAGGCTGGTGGCATTATGACGGTTGAGGACCTCAACAACTACCGTGCTGAGCTGATTGAGCATCCGATG
AGCATCGGCCTCGGTGATGCGACCCTGTACGTGCCCAGTGCCCCACTCAGCGGGCCCGTGCTGATTCTCA
TCTTGAACATCCTCAAAGGATACAACTTCTCTCCGAAGAGCGTAGCGACCCCTGAACAGAAGGCACTGAC
GTATCACCGTATCGTGGAGGCCTTTCGGTTTGCCTATGCCAAGAGGACCATGCTCGGTGACCCAAAGTTT
GTCGATGTGTCTCAGGTGATCCGCAACATGAGCTCTGAGTTCTACGCCACCCAGCTCCGAGCCCGCATCA
CTGATGAAACCACCCACCCAGCCGCCTACTATGAGCCTGAATTCTACCTTCAGGATGATGGAGGCACCGC
TCACCTGTCTGCGGTTTCAGAGGATGGCAGTGCTGTGGCTGCCACTAGTACCATCAACCTCTACTTTGGC
TCCAAGGTCCTGTCTCGGGTCAGTGGCATCCTGTTTAATGATGAGATGGATGACTTCAGCTCGCCCAACT
TCATCAACCAGTTCAGGGTAGCCCCCTCACCAGCCAACTTCATCAAGCCAGGTAAGCAGCCTCTTTCATC
CATGTGTCCCTCAATCATCCTGGATAAGGACGGCCAGGTTCGCATGGTGGTCGGAGCCTCAGGGGGTACC
CAGATCACCACATCTGTTGCACTGGCCATCATCAACAGCCTGTGGTTCGGATAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4037243), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 17215. Forward Primer - name:017215_F_IRAV20-23_L07_Ggt1, sequence:GGGTTTGGCAATAGCCCT; Reverse Primer - name:017215_R_SP6_IRAV20-23_L07_Ggt1, sequence:CGTATCCGAACCACAGGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15373 same embryo
 EMAGE:15374 same embryo
 EMAGE:15371 same embryo
 EMAGE:15375 same embryo
 EurExpress:euxassay_010561 same experiment
 MGI:4825049 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS