EMAGE mouse gene expression spatial database

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EMAGE:15375

Entpd2 ectonucleoside triphosphate diphosphohydrolase 2 ( MGI:1096863)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010575. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010575_01	euxassay_010575_02	euxassay_010575_03	euxassay_010575_04

euxassay_010575_05	euxassay_010575_06	euxassay_010575_07	euxassay_010575_08	euxassay_010575_09

euxassay_010575_10	euxassay_010575_11	euxassay_010575_12	euxassay_010575_13	euxassay_010575_14

euxassay_010575_15	euxassay_010575_16	euxassay_010575_17	euxassay_010575_18	euxassay_010575_19

euxassay_010575_20	euxassay_010575_21	euxassay_010575_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15375_wholemount_strong.wlz

15375_wholemount_moderate.wlz

15375_wholemount_weak.wlz

15375_wholemount_possible.wlz

15375_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15375_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 weak expression: see section 03 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 16 17 18 19 20 weak expression: see section 01 02 03 07 15

vagus x ganglion

moderate

regional

moderate expression: see section 14

dorsal root ganglion

moderate

regional

moderate expression: see section 03 04 05 06 12 13 14 15 weak expression: see section 07 08 09 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30781
Entity Detected:	Entpd2, ectonucleoside triphosphate diphosphohydrolase 2 ( MGI:1096863)
Sequence:	sense strand is shown >T30781 CAAGGTGCTGGAGGCAGTGACACAGACGCTCACACGGTACCCCTTTGACTTCCGTGGTGCCCGCATCCTC TCGGGACAGGATGAAGGGGTGTTTGGCTGGGTGACTGCCAACTACCTGCTGGAGAACTTCATCAAGTATG GCTGGGTAGGCCGGTGGATACGGCCAAGGAAGGGAACTCTGGGGGCCATGGACCTTGGGGGTGCCTCAAC ACAGATCACCTTTGAGACAACCAGTCCATCTGAAGATCCAGATAATGAGGTCCATTTGCGGCTCTATGGC CAGCATTACCGTGTCTACACCCATAGCTTCCTCTGCTATGGCCGGGACCAGGTTCTCCAGAGGCTTCTGG CCAGTGCCCTCCAGATCCATCGCTTCCACCCCTGCTGGCCAAAGGGCTACTCCACCCAAGTGCTGCTCCG GGAAGTCTACCAGTCTCCATGCACTATGGGTCAGCGTCCCCAGACCTTCAACAGCAGTGCCACTGTCAGC CTGTCAGGGACCAGCAACGCTGCCCTCTGTCGTGACCTCGTCTCTGGGCTCTTCAATATCTCCTCCTGTC CCTTTTCCCAATGCTCCTTCAATGGGGTTTTCCAGCCTCCCGTGGCTGGGAACTTCATAGCCTTTTCTGC TTTCTACTATACTGTAGACTTCCTGAAGACAGTGATGGGGCTGCCTGTGGGAACCCTGAAGCAGCTGGAG GATGCCACAGAGACCACCTGCAACCAGACCTGGGCTGAGCTTCAGGCCCGAGTACCCGGACAGCAGACCC GCCTGCCTGACTACTGCGCTGTAGCCATGTTCATACATCAGCTATTGAGCCGCGGTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3582550), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 10455. Forward Primer - name:010455_F_IRAV16-19_D05_Entpd2, sequence:CAAGGTGCTGGAGGCAGT; Reverse Primer - name:010455_R_SP6_IRAV16-19_D05_Entpd2, sequence:TAACCGCGGCTCAATAGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15373 same embryo
	EMAGE:15372 same embryo
	EMAGE:15374 same embryo
	EMAGE:15371 same embryo
	EurExpress:euxassay_010575 same experiment
	MGI:4824564 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source