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EMAGE:15401

Tial1 Tia1 cytotoxic granule-associated RNA binding protein-like 1 ( MGI:107913)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15401 EMAGE:15401 EMAGE:15401 EMAGE:15401 EMAGE:15401
"Pseudo-wholemount" of euxassay_010582. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010582_01 euxassay_010582_02 euxassay_010582_03 euxassay_010582_04
EMAGE:15401 EMAGE:15401 EMAGE:15401 EMAGE:15401 EMAGE:15401
euxassay_010582_05 euxassay_010582_06 euxassay_010582_07 euxassay_010582_08 euxassay_010582_09
EMAGE:15401 EMAGE:15401 EMAGE:15401 EMAGE:15401 EMAGE:15401
euxassay_010582_10 euxassay_010582_11 euxassay_010582_12 euxassay_010582_13 euxassay_010582_14
EMAGE:15401 EMAGE:15401 EMAGE:15401 EMAGE:15401 EMAGE:15401
euxassay_010582_15 euxassay_010582_16 euxassay_010582_17 euxassay_010582_18 euxassay_010582_19
EMAGE:15401 EMAGE:15401
euxassay_010582_20 euxassay_010582_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15401Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15401_wholemount_strong.wlz
15401_wholemount_moderate.wlz
15401_wholemount_weak.wlz
15401_wholemount_possible.wlz
15401_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15401_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
hypothalamus mantle layer
moderate moderate
regionalmoderate expression: see section 09 10 11 12 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30773
Entity Detected:Tial1, Tia1 cytotoxic granule-associated RNA binding protein-like 1 ( MGI:107913)
Sequence:sense strand is shown

>T30773
GCCAGTGGAGCCAAGTGTATGGAAACCCACAACAGTATGGACAGTATATGGCAAATGGGTGGCAAGTACC
ACCCTATGGAGTATATGGGCAGCCGTGGAACCAACAAGGATTTGGAGTAGATCAATCCCCTTCTGCTGCT
TGGATGGGTGGATTTGGTGCTCAGCCTCCTCAAGGACAAGCCCCTCCCCCTGTAATACCTCCTCCAAACC
AAGCTGGGTATGGAATGGCAAGCTTCCCGACACAGTGAGCTGGGACTCGAAACACGATTGTAATTCACCA
CAGGCTGCAGTCTCCTGTCACACTCTGAAGACATGAAAAGTAAACATCGGAAAATGAAAATATTTATTTT
AAAAATTGAAATGTTTGGAACCTTTAGCACAGCTTTGCTTGGTGAAGGACACATGTCTTCTAGTTCTGCC
TTTTTAAGTTTTTGTTCATGATGGATATGAACATGATTTTTCTTTGTGTACAAAAAACTAAAATAAAGTC
AATAAAGACAATTCTGACTATAAATTTTGATATAATAGGAGAAATGGGTAATACACTTCGATCCTTAGAC
ACTATTCCATTTTTATCTTGCTGTTCAGTATTTTAACTCACTGTGTTTTTAAAAGAGCAAAAAAGGGAGT
ATCGTGAAAAACTGAGAATCACATCAGTTCGTCCTGAATCCTGATACTCCCCTCCCGTCCCTGAGGCGGA
CCACATAGAAGGCAGCAGGAGAAAGTGTGATTTCAGAAGAAGTCCATGATGTGGGAGTCGCTGTGGAAGA
AATATGTTATTCTCTTATGTTAAAGAAAATGAAGCCAGACTGAAAGCGTGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3497971), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 10776. Forward Primer - name:010776_F_IRAV16-19_B15_Tial1, sequence:GCCAGTGGAGCCAAGTGT; Reverse Primer - name:010776_R_SP6_IRAV16-19_B15_Tial1, sequence:TGCACGCTTTCAGTCTGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15400 same embryo
 EMAGE:15403 same embryo
 EMAGE:15402 same embryo
 EMAGE:15405 same embryo
 EMAGE:15404 same embryo
 EurExpress:euxassay_010582 same experiment
 MGI:4828713 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS