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EMAGE:15415

Clca3 chloride channel calcium activated 3 ( MGI:1346342)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15415 EMAGE:15415 EMAGE:15415 EMAGE:15415 EMAGE:15415
"Pseudo-wholemount" of euxassay_010608. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010608_01 euxassay_010608_02 euxassay_010608_03 euxassay_010608_04
EMAGE:15415 EMAGE:15415 EMAGE:15415 EMAGE:15415 EMAGE:15415
euxassay_010608_05 euxassay_010608_06 euxassay_010608_07 euxassay_010608_08 euxassay_010608_09
EMAGE:15415 EMAGE:15415 EMAGE:15415 EMAGE:15415 EMAGE:15415
euxassay_010608_10 euxassay_010608_11 euxassay_010608_12 euxassay_010608_13 euxassay_010608_14
EMAGE:15415 EMAGE:15415 EMAGE:15415 EMAGE:15415 EMAGE:15415
euxassay_010608_15 euxassay_010608_16 euxassay_010608_17 euxassay_010608_18 euxassay_010608_19
EMAGE:15415 EMAGE:15415 EMAGE:15415
euxassay_010608_20 euxassay_010608_21 euxassay_010608_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15415Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15415_wholemount_strong.wlz
15415_wholemount_moderate.wlz
15415_wholemount_weak.wlz
15415_wholemount_possible.wlz
15415_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15415_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
bladder
strong strong
regionalExpression in the fundus region.
urethra of male
strong strong
regionalstrong expression: see section 12 13
rectum lumen
strong strong
regionalstrong expression: see section 12 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31155
Entity Detected:Clca3, chloride channel calcium activated 3 ( MGI:1346342)
Sequence:sense strand is shown

>T31155
ATGGGCCACAAGACAGGACCTTTGTCCATGAGTGGGCTCACTTCCGATGGGGAGTGTTTAATGAATACAA
CAACGACGAGAAGTTCTACTTATCCAAAGGAAAACCCCAAGCAGTGAGGTGTTCAGCAGCCATTACCGGT
AAAAATCAAGTTCGTCGGTGCCAGGGAGGCAGTTGTATCACTAACGGAAAGTGTGTAATCGACAGAGTAA
CGGGACTGTATAAAGACAATTGTGTATTTGTACCAGATCCACACCAAAACGAGAAGGCTTCCATCATGTT
TAACCAAAATATCAATTCTGTGGTTGAATTCTGTACAGAAAAAAATCACAATCAAGAAGCCCCAAATGAC
CAAAACCAACGATGCAATCTCCGAAGCACGTGGGAAGTCATCCAGGAATCTGAGGACTTCAAGCAAACCA
CTCCCATGACAGCCCAGCCACCTGCACCCACCTTCTCACTGCTGCAAATTGGACAAAGAATTGTGTGCTT
AGTTCTTGATAAGTCCGGGAGCATGCTGAACGATGATCGTCTTAACCGAATGAATCAGGCAAGCCGGCTT
TTCCTGCTGCAGACTGTGGAGCAGGGATCCTGGGTCGGGATGGTGACCTTTGACAGTGCTGCCTATGTAC
AAAGCGAACTCAAACAGTTAAACAGTGGTGCTGACAGAGATCTGCTGATCAAGCACTTACCCACAGTATC
TGCAGGAGGGACATCTATATGCTCTGGCCTTCGGACAGCATTTACAGTGATAAAGAAGAAGTATCCAACT
GATGGATCTGAAATTGTGCTGCTGACCGATGGGGAGGACAACACCATTAGCAGCTGCTTTGACCTGGTGA
AGCAGAGCGGGGCCATCATCCATAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4217548), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 53273. Forward Primer - name:053273_F_IRAV45_a02_Clca3, sequence:ATGGGCCACAAGACAGGA; Reverse Primer - name:053273_R_SP6_IRAV45_a02_Clca3, sequence:TGTATGGATGATGGCCCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15416 same embryo
 EMAGE:15412 same embryo
 EMAGE:15413 same embryo
 EMAGE:15414 same embryo
 EurExpress:euxassay_010608 same experiment
 MGI:4823894 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS