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EMAGE:15423

Slc25a4 solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 4 ( MGI:1353495)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15423 EMAGE:15423 EMAGE:15423 EMAGE:15423 EMAGE:15423
"Pseudo-wholemount" of euxassay_010636. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010636_01 euxassay_010636_02 euxassay_010636_03 euxassay_010636_04
EMAGE:15423 EMAGE:15423 EMAGE:15423 EMAGE:15423 EMAGE:15423
euxassay_010636_05 euxassay_010636_06 euxassay_010636_07 euxassay_010636_08 euxassay_010636_09
EMAGE:15423 EMAGE:15423 EMAGE:15423 EMAGE:15423 EMAGE:15423
euxassay_010636_10 euxassay_010636_11 euxassay_010636_12 euxassay_010636_13 euxassay_010636_14
EMAGE:15423 EMAGE:15423 EMAGE:15423 EMAGE:15423 EMAGE:15423
euxassay_010636_15 euxassay_010636_16 euxassay_010636_17 euxassay_010636_18 euxassay_010636_19
EMAGE:15423 EMAGE:15423 EMAGE:15423
euxassay_010636_20 euxassay_010636_21 euxassay_010636_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15423Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15423_wholemount_strong.wlz
15423_wholemount_moderate.wlz
15423_wholemount_weak.wlz
15423_wholemount_possible.wlz
15423_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15423_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31182
Entity Detected:Slc25a4, solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 4 ( MGI:1353495)
Sequence:sense strand is shown

>T31182
GGGGGATCAGGCTTTGAGCTTTCTTAAGGACTTCCTGGCAGGTGGCATCGCCGCCGCCGTCTCCAAGACG
GCGGTCGCCCCGATCGAGAGGGTCAAACTGCTGCTGCAGGTCCAGCATGCCAGCAAACAGATCAGTGCAG
AGAAGCAGTACAAAGGCATCATTGATTGTGTCGTGAGAATCCCCAAGGAGCAGGGCTTTCTCTCTTTCTG
GAGGGGTAACCTGGCCAACGTGATCCGGTACTTCCCCACTCAAGCCCTGAACTTCGCCTTCAAAGACAAG
TACAAGCAGATCTTCCTGGGAGGCGTGGATCGCCATAAGCAGTTCTGGCGCTACTTTGCTGGTAACCTGG
CCTCTGGTGGGGCAGCTGGGGCCACCTCCCTCTGCTTCGTCTACCCGCTGGACTTTGCTAGGACCAGGCT
GGCTGCCGACGTGGGCAAGGGATCTTCCCAGCGAGAATTCAATGGGCTGGGCGACTGTCTCACCAAGATC
TTCAAGTCGGACGGCCTGAAGGGTCTCTACCAGGGTTTCAGTGTCTCTGTCCAGGGCATCATCATCTACA
GAGCTGCCTACTTCGGAGTCTATGACACTGCCAAGGGGATGCTGCCAGACCCCAAGAATGTGCACATTAT
CGTGAGCTGGATGATTGCCCAGAGTGTGACAGCGGTGGCGGGGCTGGTGTCCTATCCGTTTGACACTGTT
CGTCGTAGGATGATGATGCAGTCTGGCCGGAAAGGGGCTGATATTATGTACACGGGGACACTTGACTGCT
GGAGGAAGATTGCAAAAGATGAAGGAGCCAACGCTTTCTTCAAAGGTGCTTGGTCCAATGTACTGAGAGG
CATGGGTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4500784), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 54073. Forward Primer - name:054073_F_IRAV46_f09_Slc25a4, sequence:GGGGGATCAGGCTTTGAG; Reverse Primer - name:054073_R_SP6_IRAV46_f09_Slc25a4, sequence:CCACCCATGCCTCTCAGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15427 same embryo
 EMAGE:15425 same embryo
 EMAGE:15424 same embryo
 EMAGE:15426 same embryo
 EurExpress:euxassay_010636 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS