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EMAGE:15433

Ncf1 neutrophil cytosolic factor 1 ( MGI:97283)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15433 EMAGE:15433 EMAGE:15433 EMAGE:15433 EMAGE:15433
"Pseudo-wholemount" of euxassay_010727. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010727_01 euxassay_010727_02 euxassay_010727_03 euxassay_010727_04
EMAGE:15433 EMAGE:15433 EMAGE:15433 EMAGE:15433 EMAGE:15433
euxassay_010727_05 euxassay_010727_06 euxassay_010727_07 euxassay_010727_08 euxassay_010727_09
EMAGE:15433 EMAGE:15433 EMAGE:15433 EMAGE:15433 EMAGE:15433
euxassay_010727_10 euxassay_010727_11 euxassay_010727_12 euxassay_010727_13 euxassay_010727_14
EMAGE:15433 EMAGE:15433 EMAGE:15433 EMAGE:15433 EMAGE:15433
euxassay_010727_15 euxassay_010727_16 euxassay_010727_17 euxassay_010727_18 euxassay_010727_19
EMAGE:15433 EMAGE:15433
euxassay_010727_20 euxassay_010727_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15433Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15433_wholemount_strong.wlz
15433_wholemount_moderate.wlz
15433_wholemount_weak.wlz
15433_wholemount_possible.wlz
15433_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15433_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
mandible
moderate moderate
regionalmoderate expression: see section 04 weak expression: see section 05 06 07 09 10 11 12 15 16 17 18 19 20
maxilla
weak weak
regionalweak expression: see section 06 07 10 11 12 18 19 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38305
Entity Detected:Ncf1, neutrophil cytosolic factor 1 ( MGI:97283)
Sequence:sense strand is shown

>T38305
CCTAGACGGCTCCTATCCCTATCTCTGTATATACTTGTGTATAGCCTCAGGTTAGAGACTCCTCCCTTGC
CTTAAGGTTTGGAATGGACTTAGACTCTACAGCAAAGGACAGGACTGGGTTAATCTCCACCGGTATTGCT
AGCATGAGAGGAGGCAGTGAGCGCTGCTAGGGTGGGGGTTGCAGGGACAAAAAAGATCTGATTACCTGCT
GCTGTAGTCTCGACCAAGTTTGCAGAAAGCCAAGTGCCTCAGGAACGCCCTAGGAAAGTGTTTCTCAACC
TGTTGGCCGTGACCCCTTTGGGGAGGGTGTCAAAAGACTTTCCCAGAGGTTGCCTAAGACCATTGGAAAA
TACAGATATTTATTTCTATAGATGTTTATATTATGATTCATAAAAGTAGCGAGATGACAGTTATGAAGTA
GCAATGAAAATAATTTTATGACTGGGGGTCCCCACAACATCGGGAACTAGCTGTATTAGGGTCACAGCAC
TAGGGAGGTTGAGAAGCACTGCCCTAGGGCCTCAGGATGTTGCCCGGTTGGTAGAGCTTGACTAGCATGC
AACCGGGCCTGGCTTCCATTCCCAACATCCCATAATAGGGTGTGCCAGTGTCCCAGCATTAGGGCAGTCG
AGCCGAGGCTCACATCTGTAATTTCAGAGCCAAGAGTCAAGAGAACT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 146681. Forward Primer - name:146681_F_cDNA_Ncf1, sequence:CCTAGACGGCTCCTATCCCTAT; Reverse Primer - name:146681_N_SP6_cDNA_Ncf1, sequence:AGTTCTCTTGACTCTTGGCTCTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15430 same embryo
 EMAGE:15428 same embryo
 EMAGE:15429 same embryo
 EMAGE:15431 same embryo
 EMAGE:15432 same embryo
 EurExpress:euxassay_010727 same experiment
 MGI:4826617 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS