Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:15454

Igfbp6 insulin-like growth factor binding protein 6 ( MGI:96441)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15454 EMAGE:15454 EMAGE:15454 EMAGE:15454 EMAGE:15454
"Pseudo-wholemount" of euxassay_010615. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010615_01 euxassay_010615_02 euxassay_010615_03 euxassay_010615_04
EMAGE:15454 EMAGE:15454 EMAGE:15454 EMAGE:15454 EMAGE:15454
euxassay_010615_05 euxassay_010615_06 euxassay_010615_07 euxassay_010615_08 euxassay_010615_09
EMAGE:15454 EMAGE:15454 EMAGE:15454 EMAGE:15454 EMAGE:15454
euxassay_010615_10 euxassay_010615_11 euxassay_010615_12 euxassay_010615_13 euxassay_010615_14
EMAGE:15454 EMAGE:15454 EMAGE:15454 EMAGE:15454 EMAGE:15454
euxassay_010615_15 euxassay_010615_16 euxassay_010615_17 euxassay_010615_18 euxassay_010615_19
EMAGE:15454
euxassay_010615_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15454Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15454_wholemount_strong.wlz
15454_wholemount_moderate.wlz
15454_wholemount_weak.wlz
15454_wholemount_possible.wlz
15454_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15454_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30858
Entity Detected:Igfbp6, insulin-like growth factor binding protein 6 ( MGI:96441)
Sequence:sense strand is shown

>T30858
GCAGCTGCACTGGACTCTCTGGAAGGAGTGTACAGGGCAAAAACTCTGACCATGACCTGGGATGGGCTGC
CCACGCAGCCGCTGCTCATGCTGCTAATGCTGTTGTTCGCTGCGGGCTCAGGGTCCGCCTTAGCGGGGTG
CCCCGGCTGCGGCGCTGGGATGCAGACTGGTTGTCGCGGGGGCTGCGTGGAGGAGGAAGACGCGGGATCG
CCTGCAGACGGCTGTACAGAAGCCGGAGGCTGTCTCAGGAGAGAGGGACAACCGTGCGGGGTCTACAGCC
CTAAGTGCGCCCCAGGACTGCAGTGCCAACCCCGAGAGAACGAAGAGGCGCCTTTGCGGGCGCTGCTAAT
CGGCCAGGGCCGCTGTCAACGCGCCAGAGGGCCGTCGGAGGAGACTACAAAGGAGAGCAAACCCCAAGGA
GGTGCCTCCCGCTCTCGTGACACAAACCACAGAGACCGGCAGAAGAATCCACGGACCTCTGCTGCCCCTA
TACGGCCCAATCCTGTTCAAGATAGTGAGATGGGCCCCTGCCGCAGACACTTGGATTCAGTACTGCAGCA
GCTCCAGACTGAGGTCTTCCGAGGCGGAGCACGTGGGCTCTATGTGCCAAACTGTGACCTCAGAGGCTTC
TACCGAAAGCAGCAGTGTCGTTCCTCGCAGGGGAATCGCCGTGGCCCCTGCTGGTGTGTGGATCCGATGG
GCCAGCCTTTGCCAGTGTCTCCAGATGGTCAAGGAAGCACTCAGTGCTCTGCCAGGAGCAGTGGGTGAAG
CCCCGAGGGAGCCTCCAGGACGCCAGCAGGAACAGGGGGCTGTCATTTGAGCTGTATGTGAAGCAATGAA
TAACTCTGTGCCCCAAGACCCACCTTCACGCCCCACCCCATTGTCCCTGTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4224052), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 17365. Forward Primer - name:017365_F_IRAV24-27_A23_Igfbp6, sequence:GCAGCTGCACTGGACTCTC; Reverse Primer - name:017365_R_SP6_IRAV24-27_A23_Igfbp6, sequence:TGACAGGGACAATGGGGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15457 same embryo
 EMAGE:15456 same embryo
 EMAGE:15455 same embryo
 EMAGE:15453 same embryo
 EMAGE:15452 same embryo
 EurExpress:euxassay_010615 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS