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EMAGE:15484

Ebp phenylalkylamine Ca2+ antagonist (emopamil) binding protein ( MGI:107822)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15484 EMAGE:15484 EMAGE:15484 EMAGE:15484 EMAGE:15484
"Pseudo-wholemount" of euxassay_010690. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010690_01 euxassay_010690_02 euxassay_010690_03 euxassay_010690_04
EMAGE:15484 EMAGE:15484 EMAGE:15484 EMAGE:15484 EMAGE:15484
euxassay_010690_05 euxassay_010690_06 euxassay_010690_07 euxassay_010690_08 euxassay_010690_09
EMAGE:15484 EMAGE:15484 EMAGE:15484 EMAGE:15484 EMAGE:15484
euxassay_010690_10 euxassay_010690_11 euxassay_010690_12 euxassay_010690_13 euxassay_010690_14
EMAGE:15484 EMAGE:15484 EMAGE:15484 EMAGE:15484 EMAGE:15484
euxassay_010690_15 euxassay_010690_16 euxassay_010690_17 euxassay_010690_18 euxassay_010690_19
EMAGE:15484 EMAGE:15484
euxassay_010690_20 euxassay_010690_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15484Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15484_wholemount_strong.wlz
15484_wholemount_moderate.wlz
15484_wholemount_weak.wlz
15484_wholemount_possible.wlz
15484_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15484_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
mandible
weak weak
regionalweak expression: see section 02 03 04 05 06 14 15 16 17 18 19 20
maxilla
weak weak
regionalweak expression: see section 06 14 15 16
liver lobe
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
orbito-sphenoid
weak weak
regionalweak expression: see section 02 03 04 19 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31227
Entity Detected:Ebp, phenylalkylamine Ca2+ antagonist (emopamil) binding protein ( MGI:107822)
Sequence:sense strand is shown

>T31227
TCCTGGAAAGTCGCAAGCCTGTGTTAGGAAGTCGCCCTGCGATCGCGCCGCCTGGGGTTTTTCTGTCCCT
TTGTCCTCGTTTATGTACGAAGCTGCCAGCGGGCCATAGAAACATGACCACCAATACGGTCCCCTTGCAC
CCGTACTGGCCCAGGCACCTGAAGCTGGACAACTTCGTGCCTAATGACCTCCCGACTTCGCATATCCTGG
TTGGCCTCTTCTCCATCTCTGGGGGCCTAATTGTGATCACGTGGCTGTTGTCTAGCCGAGCTTCCGTCGT
CCCACTTGGAGCTGGGCGGCGACTGGCCTTGTGCTGGTTTGCTGTGTGTACCTTCATTCACCTTGTGATC
GAGGGCTGGTTCTCTCTCTACAATGGCATCCTTTTAGAAGACCAAGCCTTCTTATCCCAACTCTGGAAAG
AGTATTCCAAGGGAGATAGCCGATATATCCTTAGTGACAGCTTCGTCGTCTGTATGGAGACTGTCACAGC
TTGTCTCTGGGGACCACTCAGCCTATGGGTAGTGATTGCCTTTCTCCGCCAACAGCCCTTCCGCTTTGTC
CTACAGCTTGTGGTGTCTATGGGCCAGATATACGGGGATGTGCTGTACTTCCTGACAGAGCTACACGAAG
GACTCCAGCATGGGGAGATAGGCCACCCCGTTTATTTCTGGTTCTATTTTGTTTTCCTGAATGCTGTATG
GTTGGTGATACCAAGCATCCTTGTGCTTGATGCCATAAAGCATCTCACTAGTGCCCAGAGCGTGCTGGAC
AGCAAAGTCATGAAAATTAAGAGCAAGCATAACTAAAGAGCCGGAGAGTGGGCTGAGTCCCTGCTGATGA
GGCGCTCTCCTCACTTCCCAGAAGACTCAAATCTTCTTCCTCCTCGCACAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3585323), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 10796. Forward Primer - name:010796_F_IRAV04-07_P06_Ebp, sequence:TCCTGGAAAGTCGCAAGC; Reverse Primer - name:010796_R_SP6_IRAV04-07_P06_Ebp, sequence:CCTGTGCGAGGAGGAAGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15487 same embryo
 EMAGE:15483 same embryo
 EMAGE:15485 same embryo
 EMAGE:15486 same embryo
 EMAGE:15482 same embryo
 EurExpress:euxassay_010690 same experiment
 MGI:4824460 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS