EMAGE mouse gene expression spatial database

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EMAGE:15497

Myo5c myosin VC ( MGI:2442485)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010683. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010683_01	euxassay_010683_02	euxassay_010683_03	euxassay_010683_04

euxassay_010683_05	euxassay_010683_06	euxassay_010683_07	euxassay_010683_08	euxassay_010683_09

euxassay_010683_10	euxassay_010683_11	euxassay_010683_12	euxassay_010683_13	euxassay_010683_14

euxassay_010683_15	euxassay_010683_16	euxassay_010683_17	euxassay_010683_18	euxassay_010683_19

euxassay_010683_20	euxassay_010683_21	euxassay_010683_22	euxassay_010683_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15497_wholemount_strong.wlz

15497_wholemount_moderate.wlz

15497_wholemount_weak.wlz

15497_wholemount_possible.wlz

15497_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15497_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 weak expression: see section 09 16 17 18

pancreas

moderate

regional

moderate expression: see section 07 08 10 weak expression: see section 09 11 12 14 15 16 17

thyroid gland

moderate

regional

moderate expression: see section 12 13 14

cochlea

weak

regional

weak expression: see section 03 04 05 06 07 08 16 17 18

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 11 12 15 16 18 19 20 weak expression: see section 10 13

esophagus

weak

regional

weak expression: see section 11

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10

rectum

moderate

regional

moderate expression: see section 12

lung

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

trachea

moderate

regional

moderate expression: see section 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31201
Entity Detected:	Myo5c, myosin VC ( MGI:2442485)
Sequence:	sense strand is shown >T31201 GCCGTGGAAGCACAGAGTGAGATGCATCCAGAAGGGAAGGAGAGGCTGGTGGGTGCGATCCACGAACCGC ATGAAGCCATCAAGTTCCCAAAGAAGCAACCAGAAGCTGAGGAGGAAGTCGAGAGTATTCTCCAACAAGA AGCGTCCCGGCTCTCTTTGGAAAAGAGGGATCTCGAGGAAGAGTTAGACATGAAGGACAGAATGATTAGA AGGCTACAAGATCAAGTCAAGACTCTGACCAAGACGATTGAGAAAGCCAACCATGTGCACCTGCCCTCGG GATCCAGGGAGTACCTGGGGATGCTGGAATACAAGAAAGAAGATGAGGGGAAGCTCATTCAAAACCTCAT TCTTGACCTGAAGCCCCGAGGTGTGGTGGTGAACATGATCCCAGGGCTGCCAGCTCACATCCTGTTCATG TGCGTGCGATACGCGGACTCCCTAAACGACGCCAACATGCTCAAATCCCTCATGAACAGTGCTATTAACG GCATCAAGCATGTGGTTAAGGAACATTTTGAAGATTTGGAGATGCTGTCCTTCTGGCTTTCCAACACTTG CCATTTTCTTAACTGCCTGAAGCAGTACAGCGGAGAAGAGGAATTCATGAAGTATAACAGCCCACAGCAG AATAAGAACTGCTTGAACAATTTTGATCTTACAGAATACAGACAGATTCTCAGTGATGTGGCCATACGGA TTTATCATCAGTTCATCATTGTGATGGAAAACAACCTCCAGCCGATAATAGTGCCAGGCATGCTGGAGTA TGAGAGCCTGCAGGGCATTTCCGGCCTGAAGCCCACTGGTTTCCGGAAGCGCTCATCCAGCATCGACGAC ACA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3491290), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 13897. Forward Primer - name:013897_F_IRAV04-07_A16_9130003O20Rik, sequence:GCCGTGGAAGCACAGAGT; Reverse Primer - name:013897_R_SP6_IRAV04-07_A16_9130003O20Rik, sequence:CTGTGTCGTCGATGCTGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15495 same embryo
	EMAGE:15498 same embryo
	EMAGE:15494 same embryo
	EMAGE:15499 same embryo
	EMAGE:15496 same embryo
	EurExpress:euxassay_010683 same experiment
	MGI:4826566 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source