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EMAGE:15499

Ube2k ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast) ( MGI:1858216)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15499 EMAGE:15499 EMAGE:15499 EMAGE:15499 EMAGE:15499
"Pseudo-wholemount" of euxassay_010681. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010681_01 euxassay_010681_02 euxassay_010681_03 euxassay_010681_04
EMAGE:15499 EMAGE:15499 EMAGE:15499 EMAGE:15499 EMAGE:15499
euxassay_010681_05 euxassay_010681_06 euxassay_010681_07 euxassay_010681_08 euxassay_010681_09
EMAGE:15499 EMAGE:15499 EMAGE:15499 EMAGE:15499 EMAGE:15499
euxassay_010681_10 euxassay_010681_11 euxassay_010681_12 euxassay_010681_13 euxassay_010681_14
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euxassay_010681_15 euxassay_010681_16 euxassay_010681_17 euxassay_010681_18 euxassay_010681_19
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euxassay_010681_20 euxassay_010681_21 euxassay_010681_22 euxassay_010681_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15499Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15499_wholemount_strong.wlz
15499_wholemount_moderate.wlz
15499_wholemount_weak.wlz
15499_wholemount_possible.wlz
15499_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15499_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31205
Entity Detected:Ube2k, ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast) ( MGI:1858216)
Sequence:sense strand is shown

>T31205
AGGAGGCGGGTACGAATCAGCTGCGGGCAGAGACATGGCCAACATCGCGGTGCAGCGAATCAAGCGGGAG
TTCAAGGAGGTGCTGAAGAGCGAGGAGACGAGCAAAAATCAAATTAAAGTAGATCTTGTAGATGAGAATT
TTACAGAATTAAGAGGAGAAATAGCAGGACCTCCAGACACACCGTATGAAGGTGGAAGATATCAACTAGA
GATAAAAATACCAGAAACATATCCATTTAACCCCCCTAAGGTCCGGTTTATCACTAAAATATGGCACCCT
AATATTAGTTCCGTCACAGGGGCTATTTGTTTGGATATCCTGAAAGATCAATGGGCAGCAGCAATGACTC
TGCGCACGGTATTATTGTCATTGCAAGCGCTGTTGGCGGCTGCAGAACCAGATGACCCCCAAGATGCAGT
AGTAGCGAATCAGTACAAACAGAATCCTGAAATGTTCAAGCAGACAGCTCGACTTTGGGCACACGTGTAC
GCTGGAGCACCAGTTTCTAGTCCAGAATACACCAAAAAAATAGAAAACCTCTGTGCTATGGGTTTTGATA
GGAACGCAGTAATAGTGGCCTTGTCTTCAAAATCATGGGATGTAGAGACTGCAACAGAACTGCTTCTGAG
TAACTGAGGCACAGGAGCTGCGCCAGCATCGCTCTCGCCTCTTGAGGAGCATCAACACCTGTTATTTTTA
GGATTCTGCATAAAGTTCTTTTAAACTGGCATTCTTGCCTAATGATGTTACCTAGGCACCATTGGAGACT
GC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3496393), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 38333. Forward Primer - name:038333_F_IRAV04-07_D01_Hip2, sequence:AGGAGGCGGGTACGAATC; Reverse Primer - name:038333_R_SP6_IRAV04-07_D01_Hip2, sequence:TGCAGTCTCCAATGGTGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15495 same embryo
 EMAGE:15498 same embryo
 EMAGE:15494 same embryo
 EMAGE:15497 same embryo
 EMAGE:15496 same embryo
 EurExpress:euxassay_010681 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS