EMAGE mouse gene expression spatial database

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EMAGE:15506

Ybx1 Y box protein 1 ( MGI:99146)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_010710_01	euxassay_010710_02	euxassay_010710_03	euxassay_010710_04	euxassay_010710_05

euxassay_010710_06	euxassay_010710_07	euxassay_010710_08	euxassay_010710_09	euxassay_010710_10

euxassay_010710_11	euxassay_010710_12	euxassay_010710_13	euxassay_010710_14	euxassay_010710_15

euxassay_010710_16	euxassay_010710_17	euxassay_010710_18	euxassay_010710_19	euxassay_010710_20

euxassay_010710_21	euxassay_010710_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15506_wholemount_strong.wlz

15506_wholemount_moderate.wlz

15506_wholemount_weak.wlz

15506_wholemount_possible.wlz

15506_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15506_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31233
Entity Detected:	Ybx1, Y box protein 1 ( MGI:99146)
Sequence:	sense strand is shown >T31233 TCCACGCAATTACCAGCAAAATTACCAGAATAGTGAGAGTGGGGAAAAGAACGAGGGATCGGAAAGCGCT CCTGAAGGCCAGGCCCAACAACGCCGGCCCTATCGCAGGCGAAGGTTCCCACCTTACTACATGCGGAGAC CCTATGCGCGTCGACCACAGTATTCCAACCCCCCTGTGCAAGGAGAAGTGATGGAGGGTGCTGACAACCA GGGTGCAGGAGAGCAAGGTAGACCAGTGAGACAGAATATGTATCGGGGTTACAGACCACGATTCCGAAGG GGCCCTCCTCGCCAAAGACAGCCTAGAGAGGATGGCAATGAAGAGGACAAAGAAAATCAAGGAGATGAGA CCCAAGGTCAGCAGCCACCTCAACGTCGGTATCGCCGAAACTTCAATTACCGACGCAGACGCCCAGAGAA CCCTAAACCACAAGATGGCAAAGAGACAAAAGCAGCCGATCCACCAGCTGAGAATTCGTCCGCTCCCGAG GCTGAGCAGGGCGGGGCTGAGTAAATGCCGGCTTACCATCTCTACCATCATCCGGTTTGGTCATCCAACA AGAAGAAATGAATATGAAATTCCAGCAATAAGAAATGAACAAAGATTGGAGCTGAAGACCTTAAGTGCTT GCTTTTTGCCCGCTGACCAGATAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4167717), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 54349. Forward Primer - name:054349_F_IRAV48_c05_Nsep1, sequence:TCCACGCAATTACCAGCA; Reverse Primer - name:054349_R_SP6_IRAV48_c05_Nsep1, sequence:GTTATCTGGTCAGCGGGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15509 same embryo
	EMAGE:15511 same embryo
	EMAGE:15508 same embryo
	EMAGE:15510 same embryo
	EMAGE:15507 same embryo
	EurExpress:euxassay_010710 same experiment
	MGI:4829251 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source