EMAGE mouse gene expression spatial database

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EMAGE:15573

Rnf217 ring finger protein 217 ( MGI:3610311)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010804. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010804_01	euxassay_010804_02	euxassay_010804_03	euxassay_010804_04

euxassay_010804_05	euxassay_010804_06	euxassay_010804_07	euxassay_010804_08	euxassay_010804_09

euxassay_010804_10	euxassay_010804_11	euxassay_010804_12	euxassay_010804_13	euxassay_010804_14

euxassay_010804_15	euxassay_010804_16	euxassay_010804_17	euxassay_010804_18	euxassay_010804_19

euxassay_010804_20	euxassay_010804_21	euxassay_010804_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15573_wholemount_strong.wlz

15573_wholemount_moderate.wlz

15573_wholemount_weak.wlz

15573_wholemount_possible.wlz

15573_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15573_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 15 16 17 18 19 20 21 22

adrenal gland

weak

regional

weak expression: see section 05 06 12 13

cerebral cortex mantle layer

weak

regional

weak expression: see section 04 06 07 08 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38385
Entity Detected:	Rnf217, ring finger protein 217 ( MGI:3610311)
Sequence:	sense strand is shown >T38385 GAATTCCTGGAGGAAACAACTGTTGTCTACAATTTAACCCATGAGGACTCTATCAAGTATAAGTACTTCT TGGAACTTGGCCGAATTGACTCCAGCACCAAGCCATGTCCTCAATGCAAACACTTCACAACCTTTAAGAA AAAAGGACATATCCCCACTCCTTCCAGATCAGAAAGCAGATACAAAATCCAGTGTCCCACTTGCCAATTG ATCTGGTGTTTTAAGTGCCACTCTCCTTGGCATGAAGGTGTTAACTGCAAGGAGTACAAAAAAGGAGACA AGTTACTGCGTCACTGGGCCAGTGAGATTGAGCACGGGCAGAGAAATGCCCAGAAGTGTCCAAAGTGCAA GATCCATATCCAGAGAACAGAAGGGTGTGACCATATGACTTGTTCACAGTGTAACACTAATTTTTGCTAT CGATGTGGAGAGAGATACCGCCAGCTCCGGTTTTTCGGAGACCACACATCAAACCTCAGTATATTTGGAT GCAAATATCGCTACCTCCCAGAGAGACCTCATTTAAGAAGATTAGTTCGAGGGTCAGTCTGTGCTGGAAA GCTCTTCATTGCGCCTCTCATCCTGGTTTTGGGATTGGCACTAGGGGCCATAGCAGTTGTAATCGGTTTA TTTGTATTTCCTATATATTGCCTTTGTAAAAAACAGAGAAAGCGATCACGGACAGGTATGCACTGGTAAT CCACAGAGGATTTCACATGATGTCAGCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 109547. Forward Primer - name:109547_F_cDNA_LOC268291, sequence:GAATTCCTGGAGGAAACAACTG; Reverse Primer - name:109547_N_SP6_cDNA_LOC268291, sequence:GGCTGACATCATGTGAAATCCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15575 same embryo
	EMAGE:15571 same embryo
	EMAGE:15574 same embryo
	EMAGE:15572 same embryo
	EMAGE:15576 same embryo
	EurExpress:euxassay_010804 same experiment
	MGI:4827774 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source