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EMAGE:15583

Pgls 6-phosphogluconolactonase ( MGI:1913421)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15583 EMAGE:15583 EMAGE:15583 EMAGE:15583 EMAGE:15583
"Pseudo-wholemount" of euxassay_010751. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010751_01 euxassay_010751_02 euxassay_010751_03 euxassay_010751_04
EMAGE:15583 EMAGE:15583 EMAGE:15583 EMAGE:15583 EMAGE:15583
euxassay_010751_05 euxassay_010751_06 euxassay_010751_07 euxassay_010751_08 euxassay_010751_09
EMAGE:15583 EMAGE:15583 EMAGE:15583 EMAGE:15583 EMAGE:15583
euxassay_010751_10 euxassay_010751_11 euxassay_010751_12 euxassay_010751_13 euxassay_010751_14
EMAGE:15583 EMAGE:15583 EMAGE:15583 EMAGE:15583 EMAGE:15583
euxassay_010751_15 euxassay_010751_16 euxassay_010751_17 euxassay_010751_18 euxassay_010751_19
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euxassay_010751_20 euxassay_010751_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15583Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15583_wholemount_strong.wlz
15583_wholemount_moderate.wlz
15583_wholemount_weak.wlz
15583_wholemount_possible.wlz
15583_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15583_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
regionalmoderate expression: see section 08 09 10
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38314
Entity Detected:Pgls, 6-phosphogluconolactonase ( MGI:1913421)
Sequence:sense strand is shown

>T38314
GTCTCATCTCGGTCTTCTCCAGCCCGCAAGAGCTGGGCGCGTCGCTGGCGCAGCTGGTGGCGCAGCGGGC
CGCGAGCTGCCTGGAAGGGGACCGTGGCCGCTTCGCGCTTGGTCTGTCAGGCGGCAGCCTGGTCTCCATG
CTAGCCCGAGACCTGCCCGCCGCCGCCGCTCCCGCTGGACCAGCCAGCTTTGCGCGCTGGACGCTCGGCT
TCTGCGACGAGCGCCTCGTGCCCTTCGACCACGCCGAGAGCACGTACGGCCTCTACCGGACACACCTGCT
CTCAAAGCTGCCCATCCCAGACAGCCAGGTCCTTACCATCAATCCTGCCCTACCTGTGGAGGATGCTGCA
GAGGACTATGCCAGGAAACTGAGACAGGCCTTGCAAGGAGACGCTGTCCCTGTGTTTGACCTGCTGATCC
TGGGAGTGGGCCCTGACGGCCATACCTGTTCGCTCTTCCCTGACCATCCCCTCCTACAGGAGCGGGAGAA
GATCGTGGCTCCCATCAGTGACTCCCCAAAGCCACCACCGCAGAGGGTGACCCTAACGCTTCCTGTGCTG
AAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 141827. Forward Primer - name:141827_F_cDNA_Pgls, sequence:GTCTCATCTCGGTCTTCTCCAG; Reverse Primer - name:141827_N_SP6_cDNA_Pgls, sequence:GTTCAGCACAGGAAGCGTTAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15580 same embryo
 EMAGE:15584 same embryo
 EMAGE:15579 same embryo
 EMAGE:15582 same embryo
 EMAGE:15581 same embryo
 EurExpress:euxassay_010751 same experiment
 MGI:4827174 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS