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EMAGE:15584

Pdcd4 programmed cell death 4 ( MGI:107490)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15584 EMAGE:15584 EMAGE:15584 EMAGE:15584 EMAGE:15584
"Pseudo-wholemount" of euxassay_010730. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010730_01 euxassay_010730_02 euxassay_010730_03 euxassay_010730_04
EMAGE:15584 EMAGE:15584 EMAGE:15584 EMAGE:15584 EMAGE:15584
euxassay_010730_05 euxassay_010730_06 euxassay_010730_07 euxassay_010730_08 euxassay_010730_09
EMAGE:15584 EMAGE:15584 EMAGE:15584 EMAGE:15584 EMAGE:15584
euxassay_010730_10 euxassay_010730_11 euxassay_010730_12 euxassay_010730_13 euxassay_010730_14
EMAGE:15584 EMAGE:15584 EMAGE:15584 EMAGE:15584 EMAGE:15584
euxassay_010730_15 euxassay_010730_16 euxassay_010730_17 euxassay_010730_18 euxassay_010730_19
EMAGE:15584 EMAGE:15584 EMAGE:15584
euxassay_010730_20 euxassay_010730_21 euxassay_010730_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15584Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15584_wholemount_strong.wlz
15584_wholemount_moderate.wlz
15584_wholemount_weak.wlz
15584_wholemount_possible.wlz
15584_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15584_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
thymus primordium
strong strong
regionalstrong expression: see section 08 09 10 11 12
epidermis
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
diencephalon lateral wall ventricular layer
strong strong
regionalstrong expression: see section 12
telencephalon ventricular layer
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
medulla oblongata floor plate
strong strong
regionalstrong expression: see section 10 11
metencephalon floor plate
strong strong
regionalstrong expression: see section 10 11
midbrain floor plate
strong strong
regionalstrong expression: see section 11
anterior naris epithelium
strong strong
regionalstrong expression: see section 13 14 17
external naris epithelium
strong strong
regionalstrong expression: see section 12 13 17 18
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16 17 18 19
esophagus epithelium
moderate moderate
regionalmoderate expression: see section 09
pharynx epithelium
moderate moderate
regionalmoderate expression: see section 10
urethra of male
strong strong
regionalstrong expression: see section 12 13 14
larynx
moderate moderate
regionalmoderate expression: see section 10 11
trachea epithelium
moderate moderate
regionalmoderate expression: see section 10
sternum
moderate moderate
regionalmoderate expression: see section 10 11
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38312
Entity Detected:Pdcd4, programmed cell death 4 ( MGI:107490)
Sequence:sense strand is shown

>T38312
CAAACTATGATGACGACCAGGAGAACTGTGTTTATGAAACTGTAGTTTTGCCCCTGGATGAGACCGCATT
TGAGAAGACTCTAACACCAATTATACAGGAATACTTTGAGCATGGAGATACAAATGAAGTTGCGGAGATG
TTAAGAGACTTAAACCTTGGGGAGATGAAGAGTGGCGTGCCGGTGTTGGCAGTGTCCTTAGCCTTGGAGG
GGAAGGCCAGCCACCGGGAGATGACATCCAAGCTGCTTTCTGACCTTTGCGGGACGGTGATGATCACAAA
TGACGTGGAAAAGTCATTTGACAAGTTGCTGAAGGATCTCCCTGAGCTAGCCTTGGACACTCCTAGGGCA
CCGCAGTTGGTGGGCCAGTTTATTGCTAGAGCTGTTGGAGATGGAATCTTATGTAATACCTATATCGATA
GTTACAAAGGAACTGTAGATTGTGTACAGGCTCGAGCTGCTCTGGATAAGGCTACTGTGCTCCTGAGTAT
GTCCAAAGGCGGGAAGCGGAAAGACAGTGTGTGGGGATCTGGAGGCGGGCAACAGCCTGTCAATCACCTT
GTTAAAGAGATTGATATGCTGCTTAAAGAGTATTTACTCTCTGGAGATATATCTGAAGCTGAACACTGCC
TTAAGGAACTGGAAGTACCTCATTTTCACCACGAGCTTGTATATGAAGCCATTATAATGGTTTTAGAGTC
AACTGGAGAAAGTGCATTCAAGATGATCTTAGATTTATTAAAATCCTTGTGGAAGTCTTCTACTATTACC
ATAGACCAAATGAAAAGGGGCTATGAGAGAATTTACAATGAAATCCCAGACATTAATCTGGATGTCCCGC
ACTCATACTCTGTTCTTGAGAGATTTGTGGAGGAATGTTTTCAGGCTGGAATAATTTCCAAACAACTCCG
TGATCTTTGTCCATCAAGGGGAAGAAAGCGTTTTGTAAGTGAAGGAGATGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 141707. Forward Primer - name:141707_F_cDNA_Pdcd4, sequence:CAAACTATGATGACGACCAGGA; Reverse Primer - name:141707_N_SP6_cDNA_Pdcd4, sequence:CCATCTCCTTCACTTACAAAACG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15580 same embryo
 EMAGE:15579 same embryo
 EMAGE:15582 same embryo
 EMAGE:15581 same embryo
 EMAGE:15583 same embryo
 EurExpress:euxassay_010730 same experiment
 MGI:4827113 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS