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EMAGE:15604

Trim26 tripartite motif-containing 26 ( MGI:1337056)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15604 EMAGE:15604 EMAGE:15604 EMAGE:15604 EMAGE:15604
"Pseudo-wholemount" of euxassay_010784. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010784_01 euxassay_010784_02 euxassay_010784_03 euxassay_010784_04
EMAGE:15604 EMAGE:15604 EMAGE:15604 EMAGE:15604 EMAGE:15604
euxassay_010784_05 euxassay_010784_06 euxassay_010784_07 euxassay_010784_08 euxassay_010784_09
EMAGE:15604 EMAGE:15604 EMAGE:15604 EMAGE:15604 EMAGE:15604
euxassay_010784_10 euxassay_010784_11 euxassay_010784_12 euxassay_010784_13 euxassay_010784_14
EMAGE:15604 EMAGE:15604 EMAGE:15604 EMAGE:15604 EMAGE:15604
euxassay_010784_15 euxassay_010784_16 euxassay_010784_17 euxassay_010784_18 euxassay_010784_19
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euxassay_010784_20 euxassay_010784_21 euxassay_010784_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15604Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15604_wholemount_strong.wlz
15604_wholemount_moderate.wlz
15604_wholemount_weak.wlz
15604_wholemount_possible.wlz
15604_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15604_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38325
Entity Detected:Trim26, tripartite motif-containing 26 ( MGI:1337056)
Sequence:sense strand is shown

>T38325
AGGTCTAAGGAACAGAGACCCCCAAGCTAAGGGTGCAATGGCAGTGTCAGCCCCCTTGAGGAGCCTGGAG
GAAGAGGTGACCTGCTCCATCTGCCTGGATTACCTGAGGGACCCAGTGACCATCGACTGTGGCCATGTCT
TCTGTCGCAGCTGCACAAGTGATATCCGCCCCATATCGGGAAACCGGCCTGTCTGCCCGCTCTGCAAGAA
GCCCTTTAAGAAGGAGAACATCCGACCCGTGTGGCAGCTAGCAAATCTGGTAGAGAACATTGAACGGCTG
AAGGTGGACAATGGCAGACAGCCAGGAGAGCTCGCCCGCGAGCCACAGGACATGAAGTTGTGTGAGCGGC
ACCAGGAGAAGCTACACTACTACTGTGAAGATGATGGCAAACTGTTGTGTGTGATGTGCAGGGAGTCCCG
GGAGCACCGCCCCCACACTGCAGTCCTGGTGGAGAAAGCCGCCCTGCCTCACAGAGAGAAAATTCTGAAC
CACTTGAACACCCTAAGGAGAGACAGAGACAAAATCCAGGGCTTTCAAGCCAAGGGAGAAGCTGATATTC
TGGCTGCACTGACAAAGCTGCAGGAGCAGAGGCAGTACATTGTGGCGGAATTTAAGCAGGGCCACCAGTT
TCTAAAGAAGCGGGAGCAGCACCTGCTAGACCAGCTGGCCACCCTGGAGCAGCTCCTCACCGAGGGCAGG
GAAAAGTTCAAGACCCGGGGCGTCAGTGAGCTTGACCGGTTGACTCTGGTCATCTCCGAGCTGGAGGGCA
AGGCACGACAGCCAGCTGCAGAGCTGATGCAGGATGTCTGCGCTACACAGGACACCAAGGACTTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 145641. Forward Primer - name:145641_F_cDNA_Trim26, sequence:AGGTCTAAGGAACAGAGACCCC; Reverse Primer - name:145641_N_SP6_cDNA_Trim26, sequence:GAAGTCCTTGGTGTCCTGTGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15602 same embryo
 EMAGE:15606 same embryo
 EMAGE:15605 same embryo
 EMAGE:15607 same embryo
 EMAGE:15603 same embryo
 EurExpress:euxassay_010784 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS