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EMAGE:15626

Lin28b lin-28 homolog B (C. elegans) ( MGI:3584032)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15626 EMAGE:15626 EMAGE:15626 EMAGE:15626 EMAGE:15626
"Pseudo-wholemount" of euxassay_010809. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010809_01 euxassay_010809_02 euxassay_010809_03 euxassay_010809_04
EMAGE:15626 EMAGE:15626 EMAGE:15626 EMAGE:15626 EMAGE:15626
euxassay_010809_05 euxassay_010809_06 euxassay_010809_07 euxassay_010809_08 euxassay_010809_09
EMAGE:15626 EMAGE:15626 EMAGE:15626 EMAGE:15626 EMAGE:15626
euxassay_010809_10 euxassay_010809_11 euxassay_010809_12 euxassay_010809_13 euxassay_010809_14
EMAGE:15626 EMAGE:15626 EMAGE:15626 EMAGE:15626 EMAGE:15626
euxassay_010809_15 euxassay_010809_16 euxassay_010809_17 euxassay_010809_18 euxassay_010809_19
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euxassay_010809_20 euxassay_010809_21 euxassay_010809_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15626Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15626_wholemount_strong.wlz
15626_wholemount_moderate.wlz
15626_wholemount_weak.wlz
15626_wholemount_possible.wlz
15626_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15626_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38395
Entity Detected:Lin28b, lin-28 homolog B (C. elegans) ( MGI:3584032)
Sequence:sense strand is shown

>T38395
GTGCCATTACTGTCAGAGCATCATGCACATGGTGGCCAACTGCCCACACAAGCTTGCCGCTCAGCTGCCC
GCCAGTTCTCAGGGAAGACAGGAGGCAGAATCCCAGCCATGCAGCTCTGCGGCACCAAGAGAAGTGGGAG
GGGGGCATGGCTGCACAGTACTGTTTCCTCAGGAGGTGAAGTCAGAAATGGCAGAGCACTCAGACAGGTC
ACCCCAAGAAGTTTCTTCCACGAAAGCGTTTGCAGCAATAGGAGAGCAAAACAAAAAGGGGCCTTTGATT
CAGAAACGGAAAAAGACTTAGTACTTGTCAGTGTTCCCTTCACCCGGGCGGGAAGTCTACCTCATGCAAG
CACAGGGGAATAGTGGTCCACAGAGAGCAGCCGGCCGGGATGTTAACTACTGCTGAGGAACTGGGAAGTT
CTTAATTAGACAAATCACTCTTAAGCAAACTACATTTAAGCAGGGTGTCATGTTTTATAGAATTGAGATA
CTACATATAGTATGTGCATAAGTGAGAGGGGAGACTTGAATCTGTATGTATGTGTGAGGATTTCACATAA
GAGTACAGGCACACACACACATATATATATACACTTTTATATATCTGTGGTTGTCTTTGTGTGTGTGTGT
GTGTGTATGTGTGTGTGTGTGTGTGTGTATGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 109582. Forward Primer - name:109582_F_cDNA_LOC380669, sequence:GTGCCATTACTGTCAGAGCATC; Reverse Primer - name:109582_N_SP6_cDNA_LOC380669, sequence:ACATACACACACACACACACACA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15630 same embryo
 EMAGE:15629 same embryo
 EMAGE:15631 same embryo
 EMAGE:15627 same embryo
 EMAGE:15628 same embryo
 EurExpress:euxassay_010809 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS