EMAGE mouse gene expression spatial database

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EMAGE:15690

Adra2a adrenergic receptor, alpha 2a ( MGI:87934)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010849. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010849_01	euxassay_010849_02	euxassay_010849_03	euxassay_010849_04

euxassay_010849_05	euxassay_010849_06	euxassay_010849_07	euxassay_010849_08	euxassay_010849_09

euxassay_010849_10	euxassay_010849_11	euxassay_010849_12	euxassay_010849_13	euxassay_010849_14

euxassay_010849_15	euxassay_010849_16	euxassay_010849_17	euxassay_010849_18	euxassay_010849_19

euxassay_010849_20	euxassay_010849_21	euxassay_010849_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15690_wholemount_strong.wlz

15690_wholemount_moderate.wlz

15690_wholemount_weak.wlz

15690_wholemount_possible.wlz

15690_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15690_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex mantle layer

moderate

regional

moderate expression: see section 10 11 17 18 weak expression: see section 12 16

not examined

regional

not examined expression: see section 10

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 11 12

pons mantle layer

moderate

single cell

moderate expression: see section 17 weak expression: see section 07 08 16

ventral grey horn

moderate

single cell

moderate expression: see section 09 13 14 weak expression: see section 11

naris

moderate

regional

moderate expression: see section 13 14

nasal capsule

moderate

regional

moderate expression: see section 15

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 14 15 weak expression: see section 10 11 12 16 17 18 19

basisphenoid bone

strong

regional

strong expression: see section 03 22 moderate expression: see section 04 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38432
Entity Detected:	Adra2a, adrenergic receptor, alpha 2a ( MGI:87934)
Sequence:	sense strand is shown >T38432 TGTGGTGTGAGATCTATTTGGCTCTCGACGTGCTCTTTTGCACGTCGTCCATAGTGCACCTGTGCGCCAT CAGCCTTGACCGCTACTGGTCCATCACGCAGGCCATCGAGTACAACCTGAAGCGCACGCCGCGTCGCATC AAGGCCATCATTGTCACCGTGTGGGTCATCTCGGCTGTCATCTCCTTCCCGCCACTCATCTCCATAGAGA AGAAGGGCGCTGGCGGCGGGCAGCAGCCGGCCGAGCCAAGCTGCAAGATCAACGACCAGAAGTGGTATGT CATCTCCTCGTCCATCGGTTCCTTCTTCGCGCCTTGCCTCATCATGATCCTGGTCTACGTGCGTATTTAC CAGATCGCCAAGCGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 150536. Forward Primer - name:150536_F_cDNA_Adra2a, sequence:TGTGGTGTGAGATCTATTTGGC; Reverse Primer - name:150536_N_SP6_cDNA_Adra2a, sequence:ACGCTTGGCGATCTGGTAAATA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15687 same embryo
	EMAGE:15689 same embryo
	EMAGE:15688 same embryo
	EMAGE:15686 same embryo
	EMAGE:15685 same embryo
	EurExpress:euxassay_010849 same experiment
	MGI:4823014 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source