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EMAGE:15731

Scn2b sodium channel, voltage-gated, type II, beta ( MGI:106921)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15731 EMAGE:15731 EMAGE:15731 EMAGE:15731 EMAGE:15731
"Pseudo-wholemount" of euxassay_010925. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010925_01 euxassay_010925_02 euxassay_010925_03 euxassay_010925_04
EMAGE:15731 EMAGE:15731 EMAGE:15731 EMAGE:15731 EMAGE:15731
euxassay_010925_05 euxassay_010925_06 euxassay_010925_07 euxassay_010925_08 euxassay_010925_09
EMAGE:15731 EMAGE:15731 EMAGE:15731 EMAGE:15731 EMAGE:15731
euxassay_010925_10 euxassay_010925_11 euxassay_010925_12 euxassay_010925_13 euxassay_010925_14
EMAGE:15731 EMAGE:15731 EMAGE:15731 EMAGE:15731 EMAGE:15731
euxassay_010925_15 euxassay_010925_16 euxassay_010925_17 euxassay_010925_18 euxassay_010925_19
EMAGE:15731
euxassay_010925_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15731Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15731_wholemount_strong.wlz
15731_wholemount_moderate.wlz
15731_wholemount_weak.wlz
15731_wholemount_possible.wlz
15731_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15731_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
facial vii ganglion
weak weak
regionalweak expression: see section 17 18
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 05
trigeminal v ganglion
weak weak
regionalweak expression: see section 04 05 06 07 08 16 17 18 19 20
vagus x ganglion
weak weak
regionalweak expression: see section 06
dorsal root ganglion
weak weak
regionalweak expression: see section 05 06 07 08 09 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39006
Entity Detected:Scn2b, sodium channel, voltage-gated, type II, beta ( MGI:106921)
Sequence:sense strand is shown

>T39006
TACTTCCACCTCCATAGCCACTGCTTCCACAAGAGACTCAGAAGGGTGAGACTCTTGAATCTGGGGTTTG
GGAAAGTGAGGGTGCAAGGGGAGGGGGAAGGTACTGCTGGGAAGGGTGCAGGGGTCAGGTAGGTTGGGCA
TGGAGTAAGGAATGATGGATAGCCAGCCAGAGAACTCCTGGGTAAGGGGAATCTAACCCTTCTGGAAGTT
CTAATCTCTTTGGCTCAGTACCTAACTGCCAAACAACACACTGCCAATGGGAGTGGGTGAGACCACCGGG
GTGCCTGGAGCAGGTGAATACTGCCCATCCTTCAAGCAAGCCAACCAGGAGTAGAGAGATGAGTTGTGGG
CTCCCCAAGCCGGCTGAAACCAAGGGTGGTGGTGTATATATAGTTCTGCAGCTTTGAGTGGCCAGTTTTC
ATCACCTGGCATGTGTCCTCTTCTTTCACAGTGCACAGACAAGGGACTTGGCTTTTGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 265477. Forward Primer - name:265477_F_cDNA_Scn2b, sequence:TACTTCCACCTCCATAGCCACT; Reverse Primer - name:265477_N_SP6_cDNA_Scn2b, sequence:GCAAAAGCCAAGTCCCTTGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15733 same embryo
 EMAGE:15734 same embryo
 EMAGE:15730 same embryo
 EMAGE:15732 same embryo
 EurExpress:euxassay_010925 same experiment
 MGI:4827902 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS