EMAGE mouse gene expression spatial database

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EMAGE:15756

Gm5514 predicted gene 5514 ( MGI:3645435)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013176. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013176_01	euxassay_013176_02	euxassay_013176_03	euxassay_013176_04

euxassay_013176_05	euxassay_013176_06	euxassay_013176_07	euxassay_013176_08	euxassay_013176_09

euxassay_013176_10	euxassay_013176_11	euxassay_013176_12	euxassay_013176_13	euxassay_013176_14

euxassay_013176_15	euxassay_013176_16	euxassay_013176_17	euxassay_013176_18	euxassay_013176_19

euxassay_013176_20	euxassay_013176_21	euxassay_013176_22	euxassay_013176_23	euxassay_013176_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15756_wholemount_strong.wlz

15756_wholemount_moderate.wlz

15756_wholemount_weak.wlz

15756_wholemount_possible.wlz

15756_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15756_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium mesenchyme

strong

regional

strong expression: see section 05 06 07 08 15 16 17 18 19

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

cerebral cortex

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

spinal cord

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

heart atrium

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21

heart ventricle

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

renal cortex

strong

regional

strong expression: see section 06 07 08 09 10 15 16 17 18 19 20

left lung

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10

right lung

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39330
Entity Detected:	Gm5514, predicted gene 5514 ( MGI:3645435)
Sequence:	sense strand is shown >T39330 GTAGTGGGCGTTGGACAAGTGGGTATGGCGTGTGCCATCAGCATTCTGGGAAAGTCTCTGGCTGATGAAC TTGCTCTGGTGGATGTGTTGGAAGACAAGCTCAAAGAAGAGATGATGGACCTGCAGCACGGGAGCTTGTT CCTCCAGACTCCGAAAATTGTGGCCGATAAAGATTACTCTGTGACAGCCAACTCTAAGATTGTGGTGGTG TCCGCAGGAGTGCGCCAGCAGGAGGGGGAGAGTCGGCTCAACCTGGTGCAGAGAAATGTCAATGTGTTCA AGTTCATCATTCCTCAGATCGTCAAATACAGCCCTGACTGCACCATCATCGTGGTTTCCAACCCAGTGGA TATTCTGACTTATGTCACCTGGAAACTGAGAGGGCTACCTAAGCACCGTGTGATTGGAAGCGGATGCAAT CTGGATTCTGCTCGATTCCGCTACCTCATGGCAGAGAAGCTTGGCATTCATCCCAGCAGCTGCCATGGAT GGATCCTGGGCGAGCATGGAGACTCCAGTGTGGCTGTGTGGAGCGGGGTGAATGTGGCAGGAGTCTCCCT CCAGGAACTGAATCCAGAAATGGGGACAGACAATGACAGTGAGAACTGGAAGGAGGTGCATAAGATGGTG GTGGTCAGTGCCTATGAAGTCATCAAGCTCAAAGGCTACACCAACTGGGCCATCGGCCTGAGCGTGGCTG ACCTCATCGAGTCCATGCTGAAAAACCTCTCCCGGATTCACCCCGTGTCTACCATGGTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 277449. Forward Primer - name:277449_F_cDNA_LOC433229, sequence:GTAGTGGGCGTTGGACAAGT; Reverse Primer - name:277449_N_SP6_cDNA_LOC433229, sequence:CACCATGGTAGACACGGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15755 same assay
	EMAGE:15757 same embryo
	EMAGE:15762 same embryo
	EMAGE:15758 same embryo
	EMAGE:15759 same embryo
	EMAGE:15760 same embryo
	EMAGE:15754 same embryo
	EurExpress:euxassay_013176 same experiment
	EMAGE:15761 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source