EMAGE mouse gene expression spatial database

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EMAGE:15801

Clec3a C-type lectin domain family 3, member a ( MGI:2685642)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013204. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013204_01	euxassay_013204_02	euxassay_013204_03	euxassay_013204_04

euxassay_013204_05	euxassay_013204_06	euxassay_013204_07	euxassay_013204_08	euxassay_013204_09

euxassay_013204_10	euxassay_013204_11	euxassay_013204_12	euxassay_013204_13	euxassay_013204_14

euxassay_013204_15	euxassay_013204_16	euxassay_013204_17	euxassay_013204_18	euxassay_013204_19

euxassay_013204_20	euxassay_013204_21	euxassay_013204_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15801_wholemount_strong.wlz

15801_wholemount_moderate.wlz

15801_wholemount_weak.wlz

15801_wholemount_possible.wlz

15801_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15801_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cervical intervertebral disc

moderate

regional

moderate expression: see section 11 12 13

lumbar intervertebral disc

moderate

regional

moderate expression: see section 11 12 13

thoracic intervertebral disc

moderate

regional

moderate expression: see section 11 12 13

sacral vertebral cartilage condensation

moderate

regional

moderate expression: see section 12 13

exoccipital bone

moderate

regional

moderate expression: see section 17 weak expression: see section 03 04 16

sternum

moderate

regional

moderate expression: see section 12

axial skeleton tail region

moderate

regional

moderate expression: see section 13 weak expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39320
Entity Detected:	Clec3a, C-type lectin domain family 3, member a ( MGI:2685642)
Sequence:	sense strand is shown >T39320 GGCTACCCATCTCGAATGAAAGCCAGGAAACACAGCAAACGCCGCGTGAAAGCAAAGGATGACGACCTGA AATCTCAAGTTGAAAAGCTGTGGCGGGAAGTCAACGCCTTGAAGGAAATGCAAGCTCTGCAGACAGTCTG TCTCCGAGGCACCAAAGTTCATAAGAAGTGCTACCTTGCTTCAGAAGGCCTCAAGCATTACCACGAAGCC AACGAAGACTGCATTTCCAAGGGAGGGACCCTGGTTGTCCCCAGGAATTCCGACGAAATCAATGCCCTTC GAGACTATGGTAAGAGGAGTCTGCCAGGTGTCAATGACTTTTGGCTAGGCATAAATGACATGGTCACAGA AGGCAAGTTCCTCGATGTCCACGGATTCGCTGTCTCCTTCCTCAACTGGGACCGTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 277909. Forward Primer - name:277909_F_cDNA_Clec3a, sequence:GGCTACCCATCTCGAATGAA; Reverse Primer - name:277909_N_SP6_cDNA_Clec3a, sequence:CACGGTCCCAGTTGAGGAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15802 same embryo
	EMAGE:15800 same embryo
	EurExpress:euxassay_013204 same experiment
	MGI:4823923 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source