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EMAGE:15908

C2cd2 C2 calcium-dependent domain containing 2 ( MGI:1891883)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15908 EMAGE:15908 EMAGE:15908 EMAGE:15908 EMAGE:15908
"Pseudo-wholemount" of euxassay_013399. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013399_01 euxassay_013399_02 euxassay_013399_03 euxassay_013399_04
EMAGE:15908 EMAGE:15908 EMAGE:15908 EMAGE:15908 EMAGE:15908
euxassay_013399_05 euxassay_013399_06 euxassay_013399_07 euxassay_013399_08 euxassay_013399_09
EMAGE:15908 EMAGE:15908 EMAGE:15908 EMAGE:15908 EMAGE:15908
euxassay_013399_10 euxassay_013399_11 euxassay_013399_12 euxassay_013399_13 euxassay_013399_14
EMAGE:15908 EMAGE:15908 EMAGE:15908 EMAGE:15908 EMAGE:15908
euxassay_013399_15 euxassay_013399_16 euxassay_013399_17 euxassay_013399_18 euxassay_013399_19
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euxassay_013399_20 euxassay_013399_21 euxassay_013399_22 euxassay_013399_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15908Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15908_wholemount_strong.wlz
15908_wholemount_moderate.wlz
15908_wholemount_weak.wlz
15908_wholemount_possible.wlz
15908_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15908_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40395
Entity Detected:C2cd2, C2 calcium-dependent domain containing 2 ( MGI:1891883)
Sequence:sense strand is shown

>T40395
GATGTACTTTCTCCAACCAGGCCACACCTCCAAATAGTGCCACTCCCTGGTTCAAGCATATACAAACCTC
CACAATCATCATCATCATCATAATTATTGTCATTATCAAGCTAAGGTTTTACTGAGTTGTTGCTTGGGCT
GCCCTGTAATTCATTCTGTAACCCAGACATATCTTGAAACTCTGATTCTCCTGCCTTTGTCTCTCAAGTT
ACAGTGAATACAGGCCTGAGCCACTGGGCCCGGAGAACTTTACTTTCAAATAAACATTTGAAAATTCTTC
ATGGGTATGGTGGTACAGGCCTGTGATCCTAGCACTTAGAAGATGGAGAGAGGAGAATGGTAGCTTCCAC
CTCAACCTGGGCTACATGAGACCCCCTTCTCAAAACAAACAACAAAAATAGAAGAAAAAGAACTGCAAAG
TTCTAGAATGATCCATCAATAATAAGTTGGTTCCCAAAGCCAGGAATCCCCACAACTTTTGGGGGATAGA
AGTGATTTTAATGCTGTGCTCTACTCGAAGCATTGCTACTCCATGCAGTCTGCAGAATTGTGATCCGTAC
CTGTCAATTCCCAGAGACTACAGGTGCCAGGAATTCTGATTCTGATGGTGCATTTTCAGTGTGGGACACA
GTAGCTGTCATCCAACATACTGGGGGCCAGAAGTGTTTCAGGCTTCTTTTGGATCTGGGGCTATCTTGAT
A
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 152782. Forward Primer - name:152782_F_cDNA_TC1413469, sequence:GATGTACTTTCTCCAACCAGGC; Reverse Primer - name:152782_N_SP6_cDNA_TC1413469, sequence:TATCAAGATAGCCCCAGATCCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15912 same embryo
 EMAGE:15907 same embryo
 EMAGE:15910 same embryo
 EMAGE:15911 same embryo
 EMAGE:15909 same embryo
 EurExpress:euxassay_013399 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS