EMAGE mouse gene expression spatial database

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EMAGE:15940

Rbm20 RNA binding motif protein 20 ( MGI:1920963)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013320. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013320_01	euxassay_013320_02	euxassay_013320_03	euxassay_013320_04

euxassay_013320_05	euxassay_013320_06	euxassay_013320_07	euxassay_013320_08	euxassay_013320_09

euxassay_013320_10	euxassay_013320_11	euxassay_013320_12	euxassay_013320_13	euxassay_013320_14

euxassay_013320_15	euxassay_013320_16	euxassay_013320_17	euxassay_013320_18	euxassay_013320_19

euxassay_013320_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15940_wholemount_strong.wlz

15940_wholemount_moderate.wlz

15940_wholemount_weak.wlz

15940_wholemount_possible.wlz

15940_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15940_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 04 16 17 18 19 20

upper leg muscle

strong

regional

strong expression: see section 02 03 04 05 06 15 16 17 18 19 20

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02 18 19 20

hand

strong

regional

strong expression: see section 02 03

lower leg rest of mesenchyme

strong

regional

strong expression: see section 01 03 04 05 06 18 19 20

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

heart atrium

strong

regional

strong expression: see section 04 05 06 07 08 09 10 12 13 14 15 16 17

heart ventricle

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16

tongue muscle

strong

regional

strong expression: see section 10 11 12 13

tail paraxial mesenchyme

strong

regional

strong expression: see section 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39969
Entity Detected:	Rbm20, RNA binding motif protein 20 ( MGI:1920963)
Sequence:	sense strand is shown >T39969 GTCAATAACCAGAACCCTCTGCTCACGTCGCCAGCCAGCGTCCAGCTGGCCCAGATACAGGCTCAGCTCA CCCTCCATCGGCTGAAGATGGCACAGACCGCAGTCACCAACAACACTGCAGCGGCCACGGTTCTGAACCA AGTCCTCTCCAAAGTGGCCATGTCCCAGCCTCTCTTCAACCAGCTCCGGCATCCGTCTGTGCTCGGCACC GCACATGGCCCTACTGGGGTGTCCCAGCATGCTGCCTCGGTTCCCAGCGCTCACTTCCCCTCAACCGCAA TCGCCTTTTCGCCCCCAAGCCAGACAGGAGGCCCAGGGCCTTCTGTGAGCCTCCCCAGCCAGCCCCCCAA TGCTATGGTAGTTCATACCTTCAGTGGAGTGGTACCTCAGACCCCTGCCCAGCCAGCTGTCATCCTTAGC CTTGGGAAGGCTGGGCCTACACCGGCTACTACAGGGTTCTATGACTATGGCAAAGCCAACTCCGGCCAAG CGTATGGTTCTGAAACGGAGGGCCAGCCAGGCTTCTTGCCAGCCTCGGCCTCAGCCACAGCATCAGGCAG TATGACCTATGAAGGGCACTATAGCCACACAGGGCAGGATGGCCAACCTGCCTTTTCTAAAGACTTCTAT GGACCCAATGCCCAAGGGCCACACATAGCAGGTGGATTTCCAGCTGATCAGACTGGGAGCATGAAGGGAG ACGTTGGTGGGCTGTTGCAAGGCACCAACAGCCAGTGGGAGAGGCCCCCTGGATTCTCAGGCCAAAACAA GCCCGATATTACAGCGGGACCCAGTTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 111597. Forward Primer - name:111597_F_cDNA_LOC433254, sequence:GTCAATAACCAGAACCCTCTGC; Reverse Primer - name:111597_N_SP6_cDNA_LOC433254, sequence:AAACTGGGTCCCGCTGTAATA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15939 same embryo
	EMAGE:15941 same embryo
	EMAGE:15938 same embryo
	EMAGE:15937 same embryo
	EMAGE:15936 same embryo
	EurExpress:euxassay_013320 same experiment
	MGI:4827651 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source