EMAGE mouse gene expression spatial database

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EMAGE:15957

Nova2 neuro-oncological ventral antigen 2 ( MGI:104296)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013411. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013411_01	euxassay_013411_02	euxassay_013411_03	euxassay_013411_04

euxassay_013411_05	euxassay_013411_06	euxassay_013411_07	euxassay_013411_08	euxassay_013411_09

euxassay_013411_10	euxassay_013411_11	euxassay_013411_12	euxassay_013411_13	euxassay_013411_14

euxassay_013411_15	euxassay_013411_16	euxassay_013411_17	euxassay_013411_18	euxassay_013411_19

euxassay_013411_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15957_wholemount_strong.wlz

15957_wholemount_moderate.wlz

15957_wholemount_weak.wlz

15957_wholemount_possible.wlz

15957_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15957_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14

diencephalon lateral wall mantle layer

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14 15

telencephalon mantle layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

medulla oblongata alar plate mantle layer

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12 13

medulla oblongata basal plate mantle layer

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12 13

rest of cerebellum mantle layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17

pons mantle layer

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14

midbrain mantle layer

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14

facial vii ganglion

moderate

regional

moderate expression: see section 15 weak expression: see section 03 04 16

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 05 06 14

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 15 weak expression: see section 03 04 05 06 07 14 16 17 18

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 05 06 14

trigeminal v nerve

weak

regional

weak expression: see section 14

spinal cord mantle layer

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13

cervico-thoracic ganglion

weak

regional

weak expression: see section 07 12

cervical ganglion

weak

regional

weak expression: see section 06 13

thoracic ganglion

weak

regional

weak expression: see section 10

dorsal root ganglion

weak

regional

weak expression: see section 04 05 06 07 08 11 12 13

neural retina

strong

regional

strong expression: see section 02 03 04 19 20

lung

moderate

regional

moderate expression: see section 04 05 11 weak expression: see section 06 07 08 10 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40035
Entity Detected:	Nova2, neuro-oncological ventral antigen 2 ( MGI:104296)
Sequence:	sense strand is shown >T40035 AATCCTTATCCCTCACCCCTTAGGATCCGCCTCCCCTGGGGGCACAGGGATCTTCTAGAAATACCTTTGC AGTTTGGCTTTGCAAAGGCCGGCCTAGGGGGCGTGGCCTCCCCATCGGAGCGCTCACTCTGACTGTCCTC CCCGTTGCCCCCACCCACTCCCCACGCCTTCATTGTAAATAGTCACTTTTGTACTTGGTTTGAGCCAGGC GACGTGGGGAGTGTCTCTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT CTCTTATTTGTGTTGGCATCTCAACTCCTGGAGGCATGGTCCACATCCCGGTTCGATTTGGGAGAAACCC AAGGTGTGCCCCGCCAAAATGTTAAGGATCTCGAGAGAACAGTATTAACTCGGACAGCTGGCTGGGGTGC ACAGGTCCTCCGACCGGGAGGAGGGAGAGAAAGCCTTTGTTCTTGAGATCCTGTGAAATGGGTTCGTTCT CCGAGCTTTCTGAAGAACCTTGGTGGAGCTGAGAATTGAATCTCTCCTCTGCTTCCTCTGTTGGAGGCTG GCTCAAAAAGAATGGCTTTGGGATGGGGCAGTCTTTGGAGAATTGACTGCTGAATTCTTCCTGGGACCTG AAGCTAACCTCTGGTAGGCTTGTTTTCTTGCAAAGAGGTAGTCAGATGAACATCCCTCTCCAACCGTTAG AGGAAAGGTCCAAGCCCCGCGGCTGGCCCTGCCTGCTGCCACTCTGCCGCATTATAGTTTGCGGGTGGGA C
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 108672. Forward Primer - name:108672_F_cDNA_LOC434131, sequence:AATCCTTATCCCTCACCCCTTA; Reverse Primer - name:108672_N_SP6_cDNA_LOC434131, sequence:GTCCCACCCGCAAACTATAATG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15956 same embryo
	EMAGE:15955 same embryo
	EMAGE:15958 same embryo
	EMAGE:15954 same embryo
	EurExpress:euxassay_013411 same experiment
	MGI:4826755 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source