EMAGE mouse gene expression spatial database

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EMAGE:15990

Mum1l1 melanoma associated antigen (mutated) 1-like 1 ( MGI:2445062)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013435. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013435_01	euxassay_013435_02	euxassay_013435_03	euxassay_013435_04

euxassay_013435_05	euxassay_013435_06	euxassay_013435_07	euxassay_013435_08	euxassay_013435_09

euxassay_013435_10	euxassay_013435_11	euxassay_013435_12	euxassay_013435_13	euxassay_013435_14

euxassay_013435_15	euxassay_013435_16	euxassay_013435_17	euxassay_013435_18	euxassay_013435_19

euxassay_013435_20	euxassay_013435_21	euxassay_013435_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15990_wholemount_strong.wlz

15990_wholemount_moderate.wlz

15990_wholemount_weak.wlz

15990_wholemount_possible.wlz

15990_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15990_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 07 08 09 10 12 14 15 16 17 18 19 20 21 22

lateral ventricle choroid plexus

weak

regional

weak expression: see section 07 08 09 10 12 13 14 15 16 17 18

metencephalon part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

tongue muscle

weak

regional

weak expression: see section 09 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45326
Entity Detected:	Mum1l1, melanoma associated antigen (mutated) 1-like 1 ( MGI:2445062)
Sequence:	sense strand is shown >T45326 GCTGCTGACATCAGTTATCCAGTCAGGAAAATCATCAAACAAGATACCTTCAGAAATATATTTCCAAAGC TATACAATGAAAACGTGGGGGAGCAGTTGCCTATGGCATCCCAAGCCAAGAGAGTATCTTTCCAGAAAAT TCTCCCTGATCGGATGAAGCCTGCTCGGGACCGAGCTAACAAGAACCTGGTAGATTTTATTGTCAATGCA AAAGGAACAGAGGACCACCTCTTGGGCATTTTAAAGGGCACCAAAAAATCCAAGTGGCTGAAATCATTTC TCAATGCAAAGAGTTTCACACCCTGTATTGAAACCTACTTTGAAGATGAAGATCAACTGGATGAGGTAGT GAAATACCTGCAAGAAATTTATAAGCAAATTGACCAGAAGATGCTGACTCTGATAAAAGATGACAAAATT AAGTTTGTCTTGGAAGTTCTTCTACCAGAAGCAATTATTTGCTCAATTTCTGCTGTTGATGGCTTAGATT ATGAGGCAGCTGAGGCAAAGTATCTAAAGGGACCATCCCTCGGCTGTAGGGAGAGAGAATTATATGATTC CAAAATCCTATTTGAAAAGAGAAGGAGGTCATTACCAAACGAAGGCCATTAATGCCAAGAGTTGGAACCA AAAATGGAAGCTAGATGAAGCTTTGGAAAAGTAATCTTCTAAAAGTCCCAATGCCTAAGTATTTGCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 210892. Forward Primer - name:210892_F_exon_9430086K21Rik, sequence:GCTGCTGACATCAGTTATCCAG; Reverse Primer - name:210892_N_SP6_exon_9430086K21Rik, sequence:GGCAAATACTTAGGCATTGGGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15994 same embryo
	EMAGE:15991 same embryo
	EMAGE:15992 same embryo
	EMAGE:15995 same embryo
	EMAGE:15993 same embryo
	EurExpress:euxassay_013435 same experiment
	MGI:4826510 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source