EMAGE mouse gene expression spatial database

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EMAGE:16004

A530013C23Rik RIKEN cDNA A530013C23 gene ( MGI:3041178)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013441. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013441_01	euxassay_013441_02	euxassay_013441_03	euxassay_013441_04

euxassay_013441_05	euxassay_013441_06	euxassay_013441_07	euxassay_013441_08	euxassay_013441_09

euxassay_013441_10	euxassay_013441_11	euxassay_013441_12	euxassay_013441_13	euxassay_013441_14

euxassay_013441_15	euxassay_013441_16	euxassay_013441_17	euxassay_013441_18	euxassay_013441_19

euxassay_013441_20	euxassay_013441_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16004_wholemount_strong.wlz

16004_wholemount_moderate.wlz

16004_wholemount_weak.wlz

16004_wholemount_possible.wlz

16004_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16004_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 02 03 04 05 06 07 15 16 17 18 19

radius

weak

regional

weak expression: see section 01

humerus

moderate

regional

moderate expression: see section 01 weak expression: see section 02 03 04 19 20 21

fibula

weak

regional

weak expression: see section 01 21

tibia

weak

regional

weak expression: see section 01 21

femur

weak

regional

weak expression: see section 02 03 04 05 17 18 19 20

meckel's cartilage

weak

regional

weak expression: see section 05 06 08 09 15 16 17 18 19

axial skeleton

weak

regional

weak expression: see section 06 07 08 12 13 14 15

scapula

moderate

regional

moderate expression: see section 19 weak expression: see section 02 03 04 18

pelvic girdle skeleton

weak

regional

weak expression: see section 07 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45405
Entity Detected:	A530013C23Rik, RIKEN cDNA A530013C23 gene ( MGI:3041178)
Sequence:	sense strand is shown >T45405 CAAAAGGGGGTTACAGACTGAGAAAAGAGAATGTTCTAATGCTGAACTCTTGGCAGGCGTCGGCTGTGGG GGGAGCCGGGGACACTCAGCTATAGTGCCTAGGAAGCCTTGTATCCCAGGTTATCAACCTGCCCCAGAAT TCATGCGCTGTGTGGCTTGGATGCTCATGGCTCCCTCTCTGGGCTTGAGCTTCCTCTGTGTGGGAAGCTT AAGGAGGCTGGCTCCCTGTCTGCCGGCTGTCCTGGCCCCTTAGGCCTCCTTGGCCACCCCCTCCATACCC TCGAGGAAGCTGAGGCTGAGGCACAGGGCCAGGGCTTGCACCTTGACAACACACCCACGCAGGGCACAAA GGCCCATTGTTTGAGCCCACGTTGGCGGGGAAGCGGGTGGGAAGCAGCCAGCCGCCTTTCACCCAACCCG CTTGTGGTCGGTGGACTGAGGAGCAATTCACAAAACTCCGCCCCTGCCTGGGCACCTGGCCAGGCCGCCT CCCGGGCAGGCACCCTTGCCAAGCTGAGCCAGCCTCTACCCTGTTCCTGGCCTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 189254. Forward Primer - name:189254_F_exon_A530013C23Rik, sequence:CAAAAGGGGGTTACAGACTGAG; Reverse Primer - name:189254_N_SP6_exon_A530013C23Rik, sequence:GAGGCCAGGAACAGGGTAGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16005 same embryo
	EMAGE:16001 same embryo
	EMAGE:16002 same embryo
	EMAGE:16003 same embryo
	EMAGE:16000 same embryo
	EurExpress:euxassay_013441 same experiment
	MGI:4822833 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source