EMAGE mouse gene expression spatial database

EMAGE:16041

Amhr2 anti-Mullerian hormone type 2 receptor ( MGI:105062)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013520. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013520_01	euxassay_013520_02	euxassay_013520_03	euxassay_013520_04

euxassay_013520_05	euxassay_013520_06	euxassay_013520_07	euxassay_013520_08	euxassay_013520_09

euxassay_013520_10	euxassay_013520_11	euxassay_013520_12	euxassay_013520_13	euxassay_013520_14

euxassay_013520_15	euxassay_013520_16	euxassay_013520_17	euxassay_013520_18	euxassay_013520_19

euxassay_013520_20	euxassay_013520_21	euxassay_013520_22

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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

urinary system

moderate

regional

moderate expression: see section 02 03 04 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45024
Entity Detected:	Amhr2, anti-Mullerian hormone type 2 receptor ( MGI:105062)
Sequence:	sense strand is shown >T45024 CTCTTTCCCAGAGAGTCCACAGGGCTGCCCAGAGAACTGCCTTTCAGCTCCCGCCTCTGCCGTTTTCCCC TGTCGGCCTCAGCAGAGTTCTTGTCTCCTCAGTGTTCAGCAAGGCCCTGGCTCTAGGAGTCCCGATCCTG TAGGCGACACTGTTCAGGTGTATGTAAATGAGTAGCTTGTTTGAACTCTCTGTACAGGCCGACATGCGTG TGGCCGACATGCCTACTGCTGTCATTCTCTGTGGGTTTCTAACC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 216552. Forward Primer - name:216552_F_exon_Amhr2, sequence:CTCTTTCCCAGAGAGTCCACAG; Reverse Primer - name:216552_N_SP6_exon_Amhr2, sequence:GGTTAGAAACCCACAGAGAATG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16042 same embryo
	EMAGE:16039 same embryo
	EMAGE:16040 same embryo
	EurExpress:euxassay_013520 same experiment
	MGI:4823110 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source