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EMAGE:16042

AI429214 expressed sequence AI429214 ( MGI:2142538)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16042 EMAGE:16042 EMAGE:16042 EMAGE:16042 EMAGE:16042
"Pseudo-wholemount" of euxassay_013518. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013518_01 euxassay_013518_02 euxassay_013518_03 euxassay_013518_04
EMAGE:16042 EMAGE:16042 EMAGE:16042 EMAGE:16042 EMAGE:16042
euxassay_013518_05 euxassay_013518_06 euxassay_013518_07 euxassay_013518_08 euxassay_013518_09
EMAGE:16042 EMAGE:16042 EMAGE:16042 EMAGE:16042 EMAGE:16042
euxassay_013518_10 euxassay_013518_11 euxassay_013518_12 euxassay_013518_13 euxassay_013518_14
EMAGE:16042 EMAGE:16042 EMAGE:16042 EMAGE:16042 EMAGE:16042
euxassay_013518_15 euxassay_013518_16 euxassay_013518_17 euxassay_013518_18 euxassay_013518_19
EMAGE:16042 EMAGE:16042
euxassay_013518_20 euxassay_013518_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16042Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16042_wholemount_strong.wlz
16042_wholemount_moderate.wlz
16042_wholemount_weak.wlz
16042_wholemount_possible.wlz
16042_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16042_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40054
Entity Detected:AI429214, expressed sequence AI429214 ( MGI:2142538)
Sequence:sense strand is shown

>T40054
CCTGTGCAGATGAGGTACTGAGTTCTGGTTCCATCAGCTTCTCTGGAGAATTACAATCCTATTCACACAC
CTCTGAGAGCCCAGTCGAGACCAAAACGCCGACTACAAGTTCGGAAGAGCAAGACGAGCAGTCAGAACTT
TCACTCTTACAGAAGGATGAAAATAAGCTGAGTGAAATGTGGATCAACCATCTCAAGAGCAAAGAAATTC
ATTCTGAAAGATCTCAACCCGACCGTAGACTCCCACCAGAAATCCCTAAGGAATCCGCAGAAGAACTGGA
TGCCCTGCAGTCTTTTTGTACTAAAAAAGTAAACTTGATTCACCAGAGACAGGATTTAAGAGCTAAGAAA
AGCAACAGACCCAAAAGGCTGCAGCTCAGATGGATTGCAGAGACCTCAGAGGTAGATGCTTTCAATTGTA
CGATCCCTGATGAACTGTGGAACAGAATCTATTTGGAAAACACGAGAGCGACTCTGGCGTACATAGGGGC
GATTACGCAACACATTTCTTCGCAGTGTCCCAGCTGTAATAGCAAAAGAGCAGAGCTGGCTCAGTCTGAC
TTCCTGAGACGAAGGAAAACTTTACTGCAGTCACTACTACTCCAAGAGAAAATAGATGAACACCTTCATA
CCACAGACTTTCTCACCCGTGTTGGAGAAGCACATCAGGGCTTCCCCAGGCTTTCAGATGATCCTAGGAT
CATCTGGAAGCGACTGACTGAGAAAATGCTGAAGGGAAGCTCCGGCTTTGGGCGGGCATATTCAAAGCAG
GTGTAGGAAGACTGTACAAGTCTTTTTAATGATTCTCAACTCACACGTCCAACCAGATAGGAGGTGCTGA
CTACTAATGATGATATGTACTATGCCCAACC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 108982. Forward Primer - name:108982_F_cDNA_LOC434302, sequence:CCTGTGCAGATGAGGTACTGAG; Reverse Primer - name:108982_N_SP6_cDNA_LOC434302, sequence:GGTTGGGCATAGTACATATCATC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16041 same embryo
 EMAGE:16039 same embryo
 EMAGE:16040 same embryo
 EurExpress:euxassay_013518 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS