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EMAGE:16044

Fndc7 fibronectin type III domain containing 7 ( MGI:2443535)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16044 EMAGE:16044 EMAGE:16044 EMAGE:16044 EMAGE:16044
"Pseudo-wholemount" of euxassay_013527. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013527_01 euxassay_013527_02 euxassay_013527_03 euxassay_013527_04
EMAGE:16044 EMAGE:16044 EMAGE:16044 EMAGE:16044 EMAGE:16044
euxassay_013527_05 euxassay_013527_06 euxassay_013527_07 euxassay_013527_08 euxassay_013527_09
EMAGE:16044 EMAGE:16044 EMAGE:16044 EMAGE:16044 EMAGE:16044
euxassay_013527_10 euxassay_013527_11 euxassay_013527_12 euxassay_013527_13 euxassay_013527_14
EMAGE:16044 EMAGE:16044 EMAGE:16044 EMAGE:16044 EMAGE:16044
euxassay_013527_15 euxassay_013527_16 euxassay_013527_17 euxassay_013527_18 euxassay_013527_19
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euxassay_013527_20 euxassay_013527_21 euxassay_013527_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16044Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16044_wholemount_strong.wlz
16044_wholemount_moderate.wlz
16044_wholemount_weak.wlz
16044_wholemount_possible.wlz
16044_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16044_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45360
Entity Detected:Fndc7, fibronectin type III domain containing 7 ( MGI:2443535)
Sequence:sense strand is shown

>T45360
CGCTTTAAAGGAGGAACTCAGACTGTTTGGAGAGGACAAGGTCAAGTGTGTCGTTGGCTGCAAAGAAGTT
CAAGATGAGCTGTGGAGAGGAGGGGACTCCACAGAGGAAAGGCCCAGAAAGTCCCCTGGAAGGTTGCCAG
ACTGAGTCAGCACACTGTCACAGCCTGGATGCATAAAAGCCACGCTTAGCAGAAACTTGTTTGGGGGCTT
CATATCCAGACACACCACAGAATGTCGCCTATCCCAAAGCCACGTAGCCCAGGCTGGCCTTGAAGTCTCA
GTCCTTCTGCCTCAACCTCCCAACGTGTCGGGTTACGGGCATCCACCATTATGCTCCATCACAAACTTTA
ATACTTCCTGGCCAGGGGTCTCTCAAAAGCTTCCCTACAGCATCCTCTCCGCAGAAGTATGGAATTGCAC
ACCCTCAGTCAAGCCATCCTCCAGAGGACAGCATTCGCCCCCAAGACAGGCCTTTGTTGAGGCCTTTAAG
CTTGAGTCTAGAGCAGCTGCTGCTAGGGCAACTGTGTGGTAACAAACGCCTTCGGCCCAGGTTCCCGGGG
AAGCTGAGCTGCCTGCAGACTTCACTGAGTCAGAGAGCGCCATGTGAGCAGACATCTGCCAGAGCCTTTG
GGAAAGCAGAAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 187854. Forward Primer - name:187854_F_exon_E230011A21Rik, sequence:CGCTTTAAAGGAGGAACTCAGA; Reverse Primer - name:187854_N_SP6_exon_E230011A21Rik, sequence:CTTCTGCTTTCCCAAAGGCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16045 same embryo
 EMAGE:16048 same embryo
 EMAGE:16047 same embryo
 EMAGE:16043 same embryo
 EMAGE:16046 same embryo
 EurExpress:euxassay_013527 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS