Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:16053

Ift140 intraflagellar transport 140 homolog (Chlamydomonas) ( MGI:2146906)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16053 EMAGE:16053 EMAGE:16053 EMAGE:16053 EMAGE:16053
"Pseudo-wholemount" of euxassay_013514. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013514_01 euxassay_013514_02 euxassay_013514_03 euxassay_013514_04
EMAGE:16053 EMAGE:16053 EMAGE:16053 EMAGE:16053 EMAGE:16053
euxassay_013514_05 euxassay_013514_06 euxassay_013514_07 euxassay_013514_08 euxassay_013514_09
EMAGE:16053 EMAGE:16053 EMAGE:16053 EMAGE:16053 EMAGE:16053
euxassay_013514_10 euxassay_013514_11 euxassay_013514_12 euxassay_013514_13 euxassay_013514_14
EMAGE:16053 EMAGE:16053 EMAGE:16053 EMAGE:16053 EMAGE:16053
euxassay_013514_15 euxassay_013514_16 euxassay_013514_17 euxassay_013514_18 euxassay_013514_19
EMAGE:16053 EMAGE:16053 EMAGE:16053
euxassay_013514_20 euxassay_013514_21 euxassay_013514_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16053Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16053_wholemount_strong.wlz
16053_wholemount_moderate.wlz
16053_wholemount_weak.wlz
16053_wholemount_possible.wlz
16053_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16053_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45009
Entity Detected:Ift140, intraflagellar transport 140 homolog (Chlamydomonas) ( MGI:2146906)
Sequence:sense strand is shown

>T45009
AAAGTATCCCAGTTTGCAGGAGGTCCCCGCCTCTGGTTATCCCCTTGGCTCATTGGCGTGGACCGTGCTA
CTGCGCCTGCTCTCCAGCGACACTCGCCGTTGGCTAACACTGGCTAAAGTGCACACGGTCACCCAGGGAA
CCCTGTGCCTAGCTGCTGCAAGCCTCTGCTGAGTACCGCTTGCAGCGGTCTCTCGAGGCGTCAACGCGCC
ACGCGCCGGGGCGGTGTCCGGTACTCAGAAGGTTCCAGAGAAGCTCTCGAGCCGCGCCTCCCCATCCGAT
GCAATGCAACTAAGCTCCGCGAGGTGCCGACTGATGGCCTGCGTGGGGTAGGCAGGATGAACTCGGGGAG
AAAAGTATTAGGAGTCTTGACGCAGCTTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 207592. Forward Primer - name:207592_F_exon_AI661311, sequence:AAAGTATCCCAGTTTGCAGGAG; Reverse Primer - name:207592_N_SP6_exon_AI661311, sequence:CAAGCTGCGTCAAGACTCCTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16049 same embryo
 EMAGE:16054 same embryo
 EMAGE:16051 same embryo
 EMAGE:16052 same embryo
 EMAGE:16050 same embryo
 EurExpress:euxassay_013514 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS