EMAGE mouse gene expression spatial database

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EMAGE:16054

Coro2a coronin, actin binding protein 2A ( MGI:1345966)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013484. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013484_01	euxassay_013484_02	euxassay_013484_03	euxassay_013484_04

euxassay_013484_05	euxassay_013484_06	euxassay_013484_07	euxassay_013484_08	euxassay_013484_09

euxassay_013484_10	euxassay_013484_11	euxassay_013484_12	euxassay_013484_13	euxassay_013484_14

euxassay_013484_15	euxassay_013484_16	euxassay_013484_17	euxassay_013484_18	euxassay_013484_19

euxassay_013484_20	euxassay_013484_21	euxassay_013484_22	euxassay_013484_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16054_wholemount_strong.wlz

16054_wholemount_moderate.wlz

16054_wholemount_weak.wlz

16054_wholemount_possible.wlz

16054_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16054_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 10 11 12 13 14

submandibular gland primordium

weak

regional

weak expression: see section 06 07 08 09 15 16 17

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 08 09 14 15

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 05 06 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 16 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 07 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 17

trigeminal v nerve

moderate

regional

moderate expression: see section 16

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 13 14 15 16

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 11 12 14 15 16 17 weak expression: see section 08 09 10

vomeronasal organ

moderate

regional

moderate expression: see section 12 14

midgut

weak

regional

weak expression: see section 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45013
Entity Detected:	Coro2a, coronin, actin binding protein 2A ( MGI:1345966)
Sequence:	sense strand is shown >T45013 CCTGAGAACTTGAGACAAGGCTTACCACAGGAGGTACCCACTCCCCCATACCTCCTGGACAAGCAGACAG AGTTCCCCCCTCCCCCCTTCCCCTCTGGACAGATAGTTTCCCCCATCACTCAGGGTCATGCTGGAGCTCA AGCGAGAATTTCAGGGGCTATCCAGGCTAACAGGTTCTGACCCACATCGTCCATTAGCACCCCACTGGAT GCACATACTGATGACCAGACCTGCCCACTAAAGCTGACCCTACAGTGGAGGGTACGGCCCTCTGAACAAG CTGCCTGAGTGGCTTCTGTTCATGGGAATGGAGACGCTTTTAGCTAGCATCCTACTGCTGTGTTGGCAAT GGAAGCAGTACAAAGCTGACTCGCACACGACCACGTCACTCACCGTCGCTGGTATCTGCACACACCAGGG TCCTGTGCTCCTTGGTTTATTCTCCATCCCTACACTACACTGGGACTCTATGCCCGGCGATGAGCTAGCT ATGCTCGCCTTCCTTGTGCTCCCGAGTATGGCAGGGATCTGGAGCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 218912. Forward Primer - name:218912_F_exon_Coro2a, sequence:CCTGAGAACTTGAGACAAGGCT; Reverse Primer - name:218912_N_SP6_exon_Coro2a, sequence:AGCTCCAGATCCCTGCCATAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16049 same embryo
	EMAGE:16051 same embryo
	EMAGE:16052 same embryo
	EMAGE:16050 same embryo
	EMAGE:16053 same embryo
	EurExpress:euxassay_013484 same experiment
	MGI:4824012 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source