EMAGE mouse gene expression spatial database

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EMAGE:16061

Mtap7d2 MAP7 domain containing 2 ( MGI:1917474)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013477. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013477_01	euxassay_013477_02	euxassay_013477_03	euxassay_013477_04

euxassay_013477_05	euxassay_013477_06	euxassay_013477_07	euxassay_013477_08	euxassay_013477_09

euxassay_013477_10	euxassay_013477_11	euxassay_013477_12	euxassay_013477_13	euxassay_013477_14

euxassay_013477_15	euxassay_013477_16	euxassay_013477_17	euxassay_013477_18	euxassay_013477_19

euxassay_013477_20	euxassay_013477_21	euxassay_013477_22	euxassay_013477_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16061_wholemount_strong.wlz

16061_wholemount_moderate.wlz

16061_wholemount_weak.wlz

16061_wholemount_possible.wlz

16061_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16061_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 15 weak expression: see section 07 08 14

pons mantle layer

moderate

regional

moderate expression: see section 06 weak expression: see section 07 16

facial vii ganglion

weak

regional

weak expression: see section 03 04 18 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 weak expression: see section 05 16 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 06 07 08 weak expression: see section 02 03 04 05 17 18 19 20 21

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06

ventral grey horn

moderate

regional

moderate expression: see section 08 weak expression: see section 07 09 10 11 12 13

dorsal root ganglion

moderate

regional

moderate expression: see section 06 weak expression: see section 07 08 09 10 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45647
Entity Detected:	Mtap7d2, MAP7 domain containing 2 ( MGI:1917474)
Sequence:	sense strand is shown >T45647 GGCCTCTCTTGGTAGAAACCTTGCGAGTTTTGCTTTGTGGGGCTTTAGCAGAGTTTCATTGTATGGTTCT CCTTATGAGCTTCAGCTGCTGCTAAAGCCCTTCCTTGACCTTCTCTACTATCATAGCCTGTGTGATGCTG TCTGGGAACCCTACTCTAGGACACTCGAATTCCCAGAGATGGAGCACAGAGACATTTAGATGTCTCTTTC TATAAAAATCTTCTATTTACCCACACTCTAGTTAAACGTGTACTACTCAACCTGGCTGCTTCTCTCCCTG TCTTTTGTGTGTGTGTATGGGGTGAGGGAAATCTAGCTACTTTCTCCCCGTCTCAGGATTCTGTTATTTT ATTATTAAGCTCCATCATCTGCAGAGCATTACTCTCCTTTACTGTTTAGGGGTTGAAGAAAGGTTTGGTT GAATGAATAAGTAAATAGTGGCAATTATTTCTTTTCCCTTATGGGAGCTGAATCTAGCAAGGACCCTTTC ATGTCACCCCTTTAAAATCGCCAAATTGTTGCTCCAAACCATTGAATGGCTTTTCAGCTGAATGAATCAA GTAACATACAGGTGGCTATTTTCATGGTTTTGCTGTTGTTTTTCTATTAGATCTTGGAATTTTCTTTTGT TTGCTCCTGCATTTGACTAACTGTA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 208797. Forward Primer - name:208797_F_exon_2900002G04Rik, sequence:GGCCTCTCTTGGTAGAAACCTT; Reverse Primer - name:208797_N_SP6_exon_2900002G04Rik, sequence:TACAGTTAGTCAAATGCAGGAGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16063 same embryo
	EMAGE:16066 same embryo
	EMAGE:16065 same embryo
	EMAGE:16064 same embryo
	EMAGE:16062 same embryo
	EurExpress:euxassay_013477 same experiment
	MGI:4826486 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source