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EMAGE:16111

Trim47 tripartite motif-containing 47 ( MGI:1917374)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16111 EMAGE:16111 EMAGE:16111 EMAGE:16111 EMAGE:16111
"Pseudo-wholemount" of euxassay_013564. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013564_01 euxassay_013564_02 euxassay_013564_03 euxassay_013564_04
EMAGE:16111 EMAGE:16111 EMAGE:16111 EMAGE:16111 EMAGE:16111
euxassay_013564_05 euxassay_013564_06 euxassay_013564_07 euxassay_013564_08 euxassay_013564_09
EMAGE:16111 EMAGE:16111 EMAGE:16111 EMAGE:16111 EMAGE:16111
euxassay_013564_10 euxassay_013564_11 euxassay_013564_12 euxassay_013564_13 euxassay_013564_14
EMAGE:16111 EMAGE:16111 EMAGE:16111 EMAGE:16111 EMAGE:16111
euxassay_013564_15 euxassay_013564_16 euxassay_013564_17 euxassay_013564_18 euxassay_013564_19
EMAGE:16111
euxassay_013564_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16111Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16111_wholemount_strong.wlz
16111_wholemount_moderate.wlz
16111_wholemount_weak.wlz
16111_wholemount_possible.wlz
16111_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16111_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
aorta
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45221
Entity Detected:Trim47, tripartite motif-containing 47 ( MGI:1917374)
Sequence:sense strand is shown

>T45221
AGTCGGGGTCTGCCTAGAGTATGCAGACCATGCCCTGGCCTTCTATGCTGTCCGAGACGGAAAGCTGAGC
CTTCTTCGGAGGCTCAAAGCCTCCCGGCCTCGCCGCAGCGGTGCTCTGGCCTCCCCCACTGACCCTTTTC
AGAGTCGCCTGGACAGCCATTTTTCCGGGCTCTTCAACCACAGGCTCAAGCCTGCCTTCTTCCTGGAGAG
TGTGGACGCCCATCTGCAGATTGGACCTCTCAAGAAATCGTGCATAACCGTGCTGAAGAGGAGGTGATGC
ACGCCCGGCTGGGTCATCCTGTGGCGCCGTTGCCCATATTTGCTAGGGGAAGGCACTAGCCTCAGAGACT
CCCACACTCCTAGCCACCTCACTTCTTGAGGCCTTCTAGACCTTCCACTTTGCAGGACTTGTTTTGAGAA
CA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 191969. Forward Primer - name:191969_F_exon_Trim47, sequence:AGTCGGGGTCTGCCTAGAGTAT; Reverse Primer - name:191969_N_SP6_exon_Trim47, sequence:TGTTCTCAAAACAAGTCCTGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16112 same embryo
 EMAGE:16109 same embryo
 EMAGE:16110 same embryo
 EMAGE:16114 same embryo
 EMAGE:16113 same embryo
 EurExpress:euxassay_013564 same experiment
 MGI:4828917 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS