EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:16112

Mark1 MAP/microtubule affinity-regulating kinase 1 ( MGI:2664902)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013555. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013555_01	euxassay_013555_02	euxassay_013555_03	euxassay_013555_04

euxassay_013555_05	euxassay_013555_06	euxassay_013555_07	euxassay_013555_08	euxassay_013555_09

euxassay_013555_10	euxassay_013555_11	euxassay_013555_12	euxassay_013555_13	euxassay_013555_14

euxassay_013555_15	euxassay_013555_16	euxassay_013555_17	euxassay_013555_18	euxassay_013555_19

euxassay_013555_20	euxassay_013555_21	euxassay_013555_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16112_wholemount_strong.wlz

16112_wholemount_moderate.wlz

16112_wholemount_weak.wlz

16112_wholemount_possible.wlz

16112_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16112_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 17 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 12 13 14 15 weak expression: see section 04 05 06

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45251
Entity Detected:	Mark1, MAP/microtubule affinity-regulating kinase 1 ( MGI:2664902)
Sequence:	sense strand is shown >T45251 ATGCACCACACCTGAGTACAGTGTCTGAATGTACTGGTGACACCTGTGTGGTCCCGCTGTGGATCGCCCA TGTAGAAATTGTCCTTAATCCAATAAGGTTATACATAGTTATGAGCTGTAAAATTCAAGTCAGTATGAGC TATAATAATCGTAGCTTCAAAAGTAGGTTCACATGTACAGGTAAGTATATTGTGTATTTCTGTTCATTTT CTGTTCATAGAGTTGTATAATAAAATATGATTGCTGTTTAAAACCTGTATAGTTGTCTAGATCTCCACAC AGAAATGTACGTTTAATGCTTTGAGTTGAAAAGGATTTCCCCTGTTATTTACATTCTGGTGTGTTTTTAA AATTCTTACCTCCATCATGCTGTTTTGAAAATTGTGTCGAGGATTAAAAGCGCACAGAAATAGTCTCTAA AATTGTAGCATGAAGTTTATAGAAATTATTTTAAACTCACATTTAATTTAAACCAGAAGTTGACAGTGGG TTGGTGTCCCGCAGTCCTCCTGCTTTCCCAGTCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 216797. Forward Primer - name:216797_F_exon_Mark1, sequence:ATGCACCACACCTGAGTACAGT; Reverse Primer - name:216797_N_SP6_exon_Mark1, sequence:AGACTGGGAAAGCAGGAGGAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16109 same embryo
	EMAGE:16111 same embryo
	EMAGE:16110 same embryo
	EMAGE:16114 same embryo
	EMAGE:16113 same embryo
	EurExpress:euxassay_013555 same experiment
	MGI:4826104 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source