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EMAGE:16131

Hydin hydrocephalus inducing ( MGI:2389007)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16131 EMAGE:16131 EMAGE:16131 EMAGE:16131 EMAGE:16131
"Pseudo-wholemount" of euxassay_013571. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013571_01 euxassay_013571_02 euxassay_013571_03 euxassay_013571_04
EMAGE:16131 EMAGE:16131 EMAGE:16131 EMAGE:16131 EMAGE:16131
euxassay_013571_05 euxassay_013571_06 euxassay_013571_07 euxassay_013571_08 euxassay_013571_09
EMAGE:16131 EMAGE:16131 EMAGE:16131 EMAGE:16131 EMAGE:16131
euxassay_013571_10 euxassay_013571_11 euxassay_013571_12 euxassay_013571_13 euxassay_013571_14
EMAGE:16131 EMAGE:16131 EMAGE:16131 EMAGE:16131 EMAGE:16131
euxassay_013571_15 euxassay_013571_16 euxassay_013571_17 euxassay_013571_18 euxassay_013571_19
EMAGE:16131 EMAGE:16131 EMAGE:16131 EMAGE:16131
euxassay_013571_20 euxassay_013571_21 euxassay_013571_22 euxassay_013571_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16131Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16131_wholemount_strong.wlz
16131_wholemount_moderate.wlz
16131_wholemount_weak.wlz
16131_wholemount_possible.wlz
16131_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16131_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
medulla oblongata part of 4th ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 06 10 11 weak expression: see section 08 09 12 13 14 15 16 17 18
diencephalon roof plate
weak weak
regionalweak expression: see section 13 14 15
choroid invagination
moderate moderate
regionalmoderate expression: see section 06 10 11 weak expression: see section 07 08 09 16 17 18
metencephalon part of 4th ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 06 10 11 weak expression: see section 08 09 12 13 14 15 16 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45322
Entity Detected:Hydin, hydrocephalus inducing ( MGI:2389007)
Sequence:sense strand is shown

>T45322
CCTAATCAGAGCCGGATACAACATCATCATCCCCTTCAAGAATGTCTTCTTGCATGCAACAAGCTTCTCC
TTCATTGTGGAGAACCCAGCCTTCAGCATCCGGGCTGCAGAGACCGTGCGGCCCAAGAAGATCAACAACA
TCACAGTCTACTTTGAGGGGAACCCGTCGGGCAGTAAAACCCCGATCACCTCTAAGTTGATTGTCACCTG
CCCTCAGTGTGAAGGCACCGAATCTGGAATCAAATGGGTTTATTATCTGAAGGGGATC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 225059. Forward Primer - name:225059_F_exon_Hydin, sequence:CCTAATCAGAGCCGGATACAAC; Reverse Primer - name:225059_N_SP6_exon_Hydin, sequence:GATCCCCTTCAGATAATAAACCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16128 same embryo
 EMAGE:16129 same embryo
 EMAGE:16130 same embryo
 EMAGE:16127 same embryo
 EMAGE:16132 same embryo
 EurExpress:euxassay_013571 same experiment
 MGI:4825490 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS